Clone and Expression of High Yield Recombinant Trehalose Synthase in Bacillus subtilis

机译：枯草芽孢杆菌的高产量重组海藻糖合酶的克隆及表达

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Trehalose synthase is one kind of intermolecular transglucosylation enzyme, which catalyzes the conversion of maltose to trehalose. In this study the trehalose synthase gene was amplified from Pseudomonas putida P06 genomic DNA, ligated with pMA5 vector, cloned into Bacillus subtilis WB800 and had good expression with the molecular weight of 77 KD. The recombinant trehalose synthase expression conditions were performed and enzyme reaction key parameters were investigated. The results showed the enzyme had optimal activity when it reacted for 2 h at 35 °C with pH value of 7.5 and the substrate concentration was 30 %. Trehalose content of samples was detected by HPLC and the enzyme activity reached to 318.12 U/ml in crude enzyme solution. This study is the first report about the expression of trehalose synthase in Bacillus subtilis, which lays the basis for trehalose large scale industrial production.

机译：海藻糖合酶是一种分子间转葡萄糖基化酶，其催化麦芽糖向海藻糖的转化。本研究从恶臭假单胞菌P06基因组DNA中扩增海藻糖合酶基因，与pMA5载体连接，克隆到枯草芽孢杆菌WB800中，表达良好，分子量为77 KD。进行了重组海藻糖合酶的表达条件，并研究了酶反应的关键参数。结果表明，该酶在35℃，pH值为7.5，底物浓度为30％的条件下反应2h时具有最佳活性。通过HPLC检测样品中的海藻糖含量，并且在粗酶溶液中酶活性达到318.12U / ml。该研究是海藻糖合酶在枯草芽孢杆菌中表达的首次报道，为海藻糖大规模工业生产奠定了基础。

著录项

来源
《International conference on applied biotechnology》|2014年|35-42|共8页
会议地点
作者
Jing Su; Chunling Ma; Tengfei Wang; Piwu Li; Ruiming Wang;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类
关键词
Trehalose synthase; Pseudomonas putida; Gene expression; Bacillus subtilis WB800;

机译：海藻糖合酶;恶臭假单胞菌;基因表达;枯草芽孢杆菌WB800;

相似文献

外文文献
中文文献
专利

1. Cloning and expression of a Trehalose Synthase from Pseudomonas putida KT2440 in Bacillus subtilis W800N for the production of TrehaloseAU Wang, Tengfei (wangtengfei1981@163.com) Dai, Kun Liu, Hongjuan Jia, Shiru Wang, Ruiming [J] . Journal of Pure & Applied Microbiology . 2014,第2期

机译：枯草芽孢杆菌W800N中恶臭假单胞菌KT2440海藻糖合酶的克隆和表达用于生产海藻糖AU Wang，Tengfei（wangtengfei1981@163.com）Dai，Liu Kun，Jia Hongjuan Jia，Wang Shiru Wang，Ruiming
2. Trehalose Production Using Recombinant Trehalose Synthase in Bacillus subtilis by Integrating Fermentation and Biocatalysis [J] . Liu Hongling, Wang Song, Song Longxiang, Journal of Agricultural and Food Chemistry . 2019,第33期

机译：通过整合发酵和生物分析，在枯草芽孢杆菌中使用重组海藻糖合酶的海藻糖生产
3. Improved Expression and Optimization of Trehalose Synthase by Regulation of Pglv in Bacillus subtilis [J] . Hongling Liu, Hao Liu, Shaojie Yang, Scientific reports. . 2019,第1期

机译：调控Pglv在枯草芽孢杆菌中改善海藻糖合酶的表达和优化
4. Clone and Expression of High Yield Recombinant Trehalose Synthase in Bacillus subtilis [C] . Jing Su, Chunling Ma, Tengfei Wang, International conference on applied biotechnology . 2014

机译：枯草芽孢杆菌中高产重组海藻糖合成酶的克隆及表达
5. Cloning and expression of the novel glgC genes from Chlamydia trachomatis and Bacillus subtilis and directed evolution of the recombinant Chlamydia ADP-glucose pyrophosphorylase. [D] . Berger, Christopher Nicholas. 2005

机译：沙眼衣原体和枯草芽孢杆菌新型glgC基因的克隆和表达，以及重组衣原体ADP-葡萄糖焦磷酸化酶的定向进化。
6. Improved Expression and Optimization of Trehalose Synthase by Regulation of Pglv in Bacillus subtilis [O] . Hongling Liu, Hao Liu, Shaojie Yang, -1

机译：调控Pglv在枯草芽孢杆菌中改善海藻糖合酶的表达和优化
7. Expression and characterization of recombinant trehalose synthase in Bacillus subtilis [O] . Nguyen Ngoc Trieu, Nguyen Thi Quynh, Nguyen Duc Hoang, 2020

机译：在枯草芽孢杆菌中重组海藻糖合酶的表达与表征< i>

8. Cloning and Expression of the Bacillus anthracis Protective Antigen Gene in Bacillus subtilis. [R] . Ivins, B. E., Welkos, S. L. 1986

机译：枯草芽孢杆菌保护性抗原基因的克隆与表达。

1. 海藻糖合酶在枯草芽孢杆菌中的高效表达 [J] . 王希晖 ,刘洪玲 ,隋松森 . 食品与发酵工业 . 2019,第007期

2. 昆明假单胞菌HL22-2海藻糖合酶基因的克隆、表达及酶学性质研究 [J] . 王一雯 ,马焕 ,权淑静 . 中国酿造 . 2020,第001期

3. 矮牵牛海藻糖-6-磷酸合酶基因PhTPS6的克隆与表达分析 [J] . 王琦 ,刘同瑞 ,刘娜 . 西北农业学报 . 2019,第002期

4. 水生栖热菌FL-03海藻糖合酶基因的克隆及真核表达 [J] . 刘俊梅 ,聂海彦 ,郑微微 . 食品科学 . 2010,第023期

5. 麦芽寡糖基海藻糖合酶基因的克隆及在大肠杆菌中的表达 [J] . 沈文 ,王金富 . 石河子大学学报（自然科学版） . 2006,第003期

6. 高效表达海藻糖合酶枯草芽孢杆菌基因工程菌株的构建及分泌表达研究 [C] . 张云霄 ,郝李振 ,王瑞明 . 第五届全国酶制剂研究开发应用技术研讨会 . 2017

7. 海藻糖合酶基因的克隆、表达及固定化研究 [A] . 黄珍 . 2018

1. 以整合型重组枯草芽孢杆菌生产海藻糖合成酶及制造海藻糖的方法 [P] . 中国专利： CN103215300B . 2015.04.08

2. 以整合型重组枯草芽孢杆菌生产海藻糖合成酶及制造海藻糖的方法 [P] . 中国专利： CN103215300A . 2013-07-24

3. NEW RECOMBINANT TREHALOSE SYNTHASE, EXPRESSION VECTOR INCLUDING ITS GENE, METHOD FOR PRODUCTION OF TREHALOSE USING RECOMBINANT TREHALOSE SYNTHASE [P] . 外国专利： KR20000037638A . 2000-07-05

机译：一种新的重组海藻糖合成酶，包括其基因的表达载体，使用重组海藻糖合成酶生产海藻糖的方法

4. Cloning vector, molecules of recombinant DNA, bacillus subtilis strains transformed with the said molecules and methods for the expression of heterologous genes and the production and secretion of proteins coded by the said genes [P] . 外国专利： US5021340A . 1991-06-04

机译：克隆载体，重组DNA分子，用所述分子转化的枯草芽孢杆菌菌株以及用于表达异源基因以及由所述基因编码的蛋白质的产生和分泌的方法

5. cloning vector, recombinant dna molecules.strains of bacillus subtilis transformed with these molecules and methods for the expression of heterologous genes and the production and secretion of proteins encoded by these genes. [P] . 外国专利： IT1186750B . 1987-12-16

机译：克隆载体，重组DNA分子，用这些分子转化的枯草芽孢杆菌菌株以及表达异源基因以及由这些基因编码的蛋白质的产生和分泌的方法。

相关主题

Clone and Expression of High Yield Recombinant Trehalose Synthase in Bacillus subtilis

摘要

著录项

相似文献

相关主题

期刊订阅