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首页> 外文会议>International conference on applied biotechnology >Effects of Glucose Assimilation on Lutein and Chlorophyll Biosyntheses in the Green Alga Chlorella pyrenoidosa
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Effects of Glucose Assimilation on Lutein and Chlorophyll Biosyntheses in the Green Alga Chlorella pyrenoidosa

机译：葡萄糖同化对绿藻小球藻叶黄素和叶绿素生物合成的影响

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Glucose assimilation caused a significant decrease of lutein and chlorophyll accumulation in Chlorella pyrenoidosa cultivated in low nitrogen medium, which are in line with the previously reported glucose-bleaching effect on the green alga Chlorella protothecoides cultivated in nitrogen deficient medium. Our study showed that an excessive lipid production induced by nitrogen deficiency was expected to be responsible for the decrease of lutein and chlorophyll accumulation in C. pyrenoidosa under heterotrophic conditions. When C. pyrenoidosa was cultivated in high nitrogen medium, the lutein and chlorophyll biosyntheses acted very differently: lutein content still decreased markedly whereas chlorophyll content remained stable. In addition, when we inhibited chlorophyll biosynthesis with levulinic acid, the chlorophyll a (chl a) and chlorophyll b (chl b) contents decreased markedly, but the lutein biosynthesis was not influenced at all. It was concluded that lutein and chlorophyll biosyntheses were possibly not coordinated in C. pyrenoidosa under heterotrophic conditions.
机译：葡萄糖同化作用导致在低氮培养基中培养的小球藻中叶黄素和叶绿素积累的显着降低，这与先前报道的对在缺氮培养基中培养的绿藻原球藻的葡萄糖漂白作用相一致。我们的研究表明，在缺养条件下，氮缺乏引起的过多脂质生产有望导致叶黄体中叶黄素和叶绿素积累的下降。当在高氮培养基中培养拟南芥时，叶黄素和叶绿素的生物合成有很大不同：叶黄素含量仍显着下降，而叶绿素含量保持稳定。此外，当我们用乙酰丙酸抑制叶绿素的生物合成时，叶绿素a（chl a）和叶绿素b（chl b）含量显着降低，但叶黄素的生物合成完全没有受到影响。结论是，在异养条件下，C.pyrenoidosa中的叶黄素和叶绿素生物合成可能不协调。

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