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首页> 外文会议>Canadian Society for Civil Engineering annual conference >A NEW HIGH-YIELDING BIO-DISPERSANT PRODUCER MUTATED FROM RHODOCOCCUS ERYTHROPOLIS STRAIN P6-4P
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A NEW HIGH-YIELDING BIO-DISPERSANT PRODUCER MUTATED FROM RHODOCOCCUS ERYTHROPOLIS STRAIN P6-4P

机译：一种由红球菌红球菌P6-4P诱变的新型高产生物分散剂

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Preeminent effectiveness and feasibility of dispersants have been the key reasons for their widely serving as the response agents in oil spill responses. Moreover, dispersants can also overcome the limitation factors of other countermeasures like accessibility, weather conditions, sea states, and oil thickness. However, the public concerns of the usages of the chemically synthetic dispersants are also essential due to their toxicity and persistency in the ecosystem. Bio-dispersants can be a promising alternative as the proven features of lower toxicity and persistency while with high effectiveness, but its broad application prospects are currently restricted by the high production cost that is 3-10 times more than chemical synthetic ones because of the low productivity. Thus, a hyper bio-dispersant producer will be the desired coping strategy. An isolated bio-dispersant producer from NL offshore, Rhodococcus erythropolis strain P6-4P was selected for generating high-yielding producers by mutation. After UV mutagenesis, 21 enhanced mutants were selected through oil spreading screening method. Further productivity quantify test of critical micelle dilution (CMD) with higher resolution was conducted to these mutants. An outstanding mutant showed CMD as high as 225 while 15.4 is the CMD of the wild type strain, which means the new mutant is 14.6 times increase. The 16S rDNA sequencing results revealed that the 16 S ribosomal DNA of the mutant 100% matched with the original strain indicating the mutation occurred on other parts of the genome which will be identified through next-generation sequencing and comparative analysis in the future study. This mutated high-yielding strain was capable to significantly improve the production rate and the total yield of bio-dispersants. The yield of crude bio-dispersant was 54g per liter with 6 days incubation. At 4mg/uL crude product/crude oil ratio, the dispersion effectiveness was found comparable to Corexit 9500A at 1:25 (dispersant/crude oil ratio). Future works on further mutagenesis base on this new high-producing strain by novel mutation methods were also discussed.
机译：分散剂出色的有效性和可行性一直是其广泛用作溢油应急响应剂的关键原因。此外，分散剂还可以克服其他对策的限制因素，例如可及性，天气状况，海况和油厚。然而，由于化学合成分散剂的毒性和在生态系统中的持久性，公众对其使用的关注也至关重要。生物分散剂由于具有较低的毒性和持久性，同时具有很高的功效，因此已被证明是一种有前途的替代品，但由于其生产成本低，其目前的广泛应用前景受到生产成本的限制，该生产成本是化学合成产品的3-10倍以上生产率。因此，高生物分散剂生产商将是理想的应对策略。选择了来自NL近海的分离的生物分散剂生产者红球菌P6-4P菌株，以通过突变产生高产的生产者。紫外诱变后，通过涂油筛选方法筛选出21个增强突变体。对这些突变体进行了更高分辨率的临界胶束稀释度（CMD）的进一步生产力定量测试。一个优秀的突变体显示CMD高达225，而野生型菌株的CMD则为15.4，这意味着新突变体的CMD增长了14.6倍。 16S rDNA测序结果表明，该突变体的16 S核糖体DNA与原始菌株100％匹配，表明该突变发生在基因组的其他部分，将在下一代研究中通过下一代测序和比较分析进行鉴定。这种突变的高产菌株能够显着提高生物分散剂的生产率和总产率。温育6天时，粗生物分散剂的产量为54g /升。在原油/原油比为4mg / uL时，发现在1:25（分散剂/原油比）下的分散效果可与Corexit 9500A媲美。还讨论了通过这种新型高产菌株通过新型突变方法进一步诱变的未来工作。

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