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首页> 外文会议>National Conference on Biomedical Engineering;International Iranian Conference on Biomedical Engineering >Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release
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Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release

机译：用于持续释放蛋白质的透明质酸-酪胺微凝胶的合成

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Microgels are hydrophilic polymer matrix with high water content suitable for encapsulation and delivery of biomolecules. In this study, hyaluronic acid (HA) microgels were synthesized using a water in oil emulsion method. First, hyaluronic acid was modified with tyramine, and then the microemulsion was produced by homogenizing the polymer solution in isooctane as an oil phase. HA microdroplets were crosslinked via enzymatic method by addition of horseradish peroxidase (enzyme) and hydrogen peroxide, and stable microgels were produced in a mild crosslinking reaction. According to the results, larger microgels were achieved by increasing the initial polymer concentration. Two sample proteins, Bovine serum albumin (BSA) and lysozyme, were incorporated in the polymer network to investigate the encapsulation efficiency of the microgels. The results demonstrated that the proposed method has a high efficiency for protein encapsulation (> 70%). The release profiles showed that lysozyme, as a cationic protein, was released in a sustained manner over a period of two weeks. However, BSA, as a negatively charged protein, showed a faster release rate. The simple method of microgel fabrication, besides the sustained release of the encapsulated proteins, makes the HA microgels a promising vehicle for delivery of cationic proteins.
机译：微凝胶是具有高水含量的亲水性聚合物基质，适合于生物分子的包封和递送。在这项研究中，使用油包水乳液法合成了透明质酸（HA）微凝胶。首先，用酪胺修饰透明质酸，然后通过将聚合物溶液在异辛烷中均质化为油相来制备微乳液。通过添加辣根过氧化物酶（酶）和过氧化氢，通过酶促方法使HA微滴交联，并在温和的交联反应中产生稳定的微凝胶。根据结果​​，通过增加初始聚合物浓度可获得更大的微凝胶。将两种样品蛋白，牛血清白蛋白（BSA）和溶菌酶，掺入了聚合物网络中，以研究微凝胶的包封效率。结果表明，所提出的方法具有很高的蛋白质封装效率（> 70％）。释放曲线表明溶菌酶，作为一种阳离子蛋白，在两周内持续释放。但是，BSA作为带负电荷的蛋白质，显示出更快的释放速率。除了持续释放被包封的蛋白质之外，微凝胶制备的简单方法使HA微凝胶成为递送阳离子蛋白质的有前途的载体。

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