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首页> 外文会议>Joint annual meeting of the International Society of Exposure Science and the International Society for Environmental Epidemiology >Distinct Fecal Microbiota and Serum Metabolite Profiles in Male Rat Exposed to Aluminum Oxide and Aluminum Oxide Nanoparticles
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Distinct Fecal Microbiota and Serum Metabolite Profiles in Male Rat Exposed to Aluminum Oxide and Aluminum Oxide Nanoparticles

机译：暴露于氧化铝和氧化铝纳米颗粒的雄性大鼠的粪便微生物群和血清代谢产物特征

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The widely application of aluminum oxide nanoparticles (AI203 NPs) in industry and personal care products has been raising concerns about their potential adverse consequences. However, the knowledge on their influence on gut microbiota and serum metabolism remains poorly understood. This study employed an integrated approach combining 16S rRNA gene sequencing and untargeted metabolomics profiling to investigate the impact of a 28 days oral exposure to a- and y-AI203 NPs and the equivalent dose of bulk AI203 on the toxic response, fecal microbiota composition and serum metabolites of male rats. At the equal dose of 100 mg/kg bw, ct-AI203 NPs had a greater effect on white blood cell count, percent of monocytes, liver SOD activity and plasma GSH content, than that of y-AI203 NPs and bulk AI203.16S results revealed NPs exposure significantly perturbed the fecal microbiome composition, and the relative abundance of genus such as Desulfovibrio, Steroidobacter and Acidibacter was significantly reduced. A significantly lower alpha diversity and distinct microbial communities, as reflected by beta diversity, were observed in ct-AI203 NPs treatment, compared to bulk AI203 and control. UHPIC-QTOFMS indicated that the circulating serum metabolites (e.g. 3-hydroxyisovaleric acid, dopamine, D-glucuronate) were significantly more abundant with a-AI203 NPs, while metabolites (e.g. corticosterone, glycerol, glycerol 3-phosphate) were significantly induced by y-AI203 NPs. Pathway analysis further indicated potential defects in the D-glutamine and D-glutamate metabolism and phenylalanine, tyrosine and tryptophan biosynthesis in a-AI203 NPs, while glycerolipid and linoleic acid metabolism were significantly affected by y-AI203 NPs. This study provides novel insights regarding the differential fecal microbiome and serum metabolome affected by nanoparticulate forms versus bulk forms of aluminum oxide in organisms.
机译：氧化铝纳米颗粒（AI203 NPs）在工业和个人护理产品中的广泛应用引起了人们对其潜在不良后果的担忧。然而，关于它们对肠道菌群和血清代谢的影响的知识仍然知之甚少。这项研究采用了结合16S rRNA基因测序和非靶向代谢组学分析的综合方法，研究了28天口服a-和y-AI203 NPs和当量的大量AI203对毒性反应，粪便微生物群组成和血清的影响雄性大鼠的代谢产物。在100 mg / kg bw的等剂量剂量下，ct-AI203 NP对白细胞计数，单核细胞百分比，肝脏SOD活性和血浆GSH含量的影响要大于y-AI203 NP和大量AI203.16S的结果NPs暴露显着干扰了粪便微生物组的组成，而Desulfovibrio，Sterroidobacter和Acidibacter等属的相对丰度却大大降低了。与大量的AI203和对照组相比，在ct-AI203 NPs处理中观察到的β多样性显着降低了α多样性和独特的微生物群落。 UHPIC-QTOFMS表明，α-Al2O3NPs使循环血清代谢产物（例如3-羟基异戊酸，多巴胺，D-葡萄糖醛酸酯）明显更丰富，而y诱导了代谢产物（例如皮质酮，甘油，3-磷酸甘油） -AI203 NP。途径分析进一步表明，a-AI203 NPs中D-谷氨酰胺和D-谷氨酸代谢以及苯丙氨酸，酪氨酸和色氨酸生物合成中存在潜在缺陷，而y-AI203 NPs显着影响甘油脂和亚油酸代谢。这项研究提供了关于生物体内不同形式的粪便微生物组和血清代谢组受到纳米颗粒形式与散装形式氧化铝的影响的新颖见解。

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