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Monolayer spectroscopy of live standard cells - light transmission and adsorption

机译：Live标准电池的单层光谱 - 光传输和吸附

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Photons irradiated into biological materials show interactions with cells, molecules and molecule structures, the amount of interaction being dependent on wavelength, energy density, radiation duration and properties of biological materials. Soft tissue is known to have photons strongly forward scattered, because of the inhomogeneity of its cellular and subcellular structures. The geometric dimensions of the scattering centers are in the order of wavelengths (Mie-scattering). Investigations described in this paper will show the influence of photons on single, live standard cells and how the light transmission through a monolayer cell will change within a measuring time of up to one hour and in dependence on cell intensity. Experiments were carried out in vivo with monolayers of standard bovine kidney cells by means of optical spectroscopy in a range of wavelengths from 200 nm to 1.500 nm. Cell cultures as mono cell layers in chambers of glasses were developed by a nutrient solution within 3 days. The growth of the cell layers was microscopically controlled. Details are described in the paper. Spectrophotometric measurements in visual- and near infrared range were carried out with cells on chambers of glasses (in nutrient solutions). Various effects, such as colour of the nutrient solution or dying of cell regions will influence the measuring results. The measurements allow conclusions as to the determination of optimal wavelengths for an optical inspection of tissue. A photon scattering is possible preferably at the boundaries (cell faces, nuclear membrane, alteration of refractive index).
机译：照射到生物材料中的光子显示与细胞，分子和分子结构的相互作用，相互作用的量取决于生物材料的波长，能量密度，辐射持续时间和性能。已知软组织具有强烈向前散射的光子，因为其细胞和亚细胞结构的不均匀性。散射中心的几何尺寸是波长（mie散射）的顺序。本文描述的研究将显示光子对单个，实时标准电池的影响以及通过单层电池的光传输如何在高达1小时的测量时间内变化，并且依赖于电池强度。通过光谱法在50nm至1.500nm的波长范围内的光谱法在体内用标准牛肾细胞进行实验。作为玻璃腔室中的单细胞层的细胞培养物在3天内通过营养溶液开发。细胞层的生长是显微镜控制的。本文描述了细节。在玻璃腔室（以营养溶液中的腔室中的细胞进行分光光度测量。各种效果，例如营养溶液的颜色或细胞区域的染色将影响测量结果。测量允许结论确定组织的光学检查的最佳波长。可以优选地在边界（细胞面，核膜，折射率的变化）中进行光子散射。

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