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Next Generation Diet Formulation: True Metabolic Availability of Amino Acids in Diets for Pigs

机译:下一代饮食制剂:猪饮食中氨基酸的真正代谢可用性

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Not all of the dietary amino acid that is included in standardized ileal digestibility is metabolically available for protein synthesis. A rapid method was developed to determine metabolic amino acid availability in feedstuffs for protein synthesis. Using the indicator amino acid oxidation technique, a change in phenylalanine oxidation, which is inversely proportional to protein synthesis, was used to measure the metabolic availability of lysine in feedstuffs compared to that of free lysine, which isfully available. Data regarding several methodological considerations were addressed before this technique was validated, including: adaptation time between test diets, repeatability of measurements, predictability of response to lysine intake, dietaryphenylalanine availability and lysine intake sensitivities. In addition, an oral dosing regimen was developed to simplify the facilities required and lower costs further. This isotope dilution method is suitable for the determination of the metabolic availability of amino acids for protein synthesis in a given feedstuff within 2-3 weeks. This method could also be adapted to predict the availability of other economically important amino acids in feedstuffs (ie threonine, methionine, tryptophan). This new approach is an improvement over the chemical methods which are only available for lysine.
机译:并非所有包含在标准化髂骨消化率中的膳食氨基酸在代谢上可用于蛋白质合成。开发了一种快速方法以确定蛋白质合成饲料中的代谢氨基酸可用性。使用指标氨基酸氧化技术,与蛋白质合成成反比,与蛋白质合成成反比的苯丙氨酸氧化的变化用于测量饲料中赖氨酸的代谢可用性,与游离赖氨酸相比,其特殊地可用。在验证该技术之前解决了关于多种方法考虑的数据,包括:试尿中的适应时间,测量的可重复性,对赖氨酸摄入的可预测性,膳食酰亚胺可用性和赖氨酸进气敏性。此外,开发了口服给药方案,以简化所需的设施,进一步降低成本。该同位素稀释方法适用于在2-3周内确定在给定饲料中蛋白质合成的氨基酸的代谢可用性。该方法还可以适用于预测饲料中其他经济上重要氨基酸的可用性(即苏氨酸,甲硫氨酸,色氨酸)。这种新方法是对仅适用于赖氨酸的化学方法的改进。

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