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首页> 外文会议>Asian conference on plant pathology >GMO components were detected from imported products by a new reliable method-Capture-PCR-ELISA
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GMO components were detected from imported products by a new reliable method-Capture-PCR-ELISA

机译：通过一种新的可靠方法 - 捕获 - PCR-ELISA从进口产品中检测到GMO组分

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摘要

The biosafety of genetically modified organisms (GMOs), especially foods with GMOs components and living GMOs in the environment is widely concerned all over the world. The first commercial available GMO crop is FLAVR-SAVR tomato. The most important commercial GMO crops are corn, soybean, cotton, rapeseed, potato, tobacco, squash, papaya, chicory. Nowadays, almost all the important crops are used to be artificially modified genetically. The methods used to identify the GMO products in the international transportation should be efficient, sensitive, time-saving, and accurate. The PCR is widely used in this field. The key factors reducing PCR sensitivity and assurance are the preparation of the high purity sample nucleic acids and discrimination of the target amplified products from non-specifically amplified molecules. Up to today there are no methods, which can be used widely to prepare nucleic acids for PCR from all the most important plants. A reliable new method, Capture-PCR-ELISA (cPCR-ELISA), was developed for the detection of GMOs. This method may be used widely, and be a resolution for the above mentioned PCR limited factors.
机译：基因改性生物（GMOS）的生物安全，特别是具有GMOS组件和环境中的生物转基因生物的食物在世界各地广泛关注。第一个可商业的GMO作物是Flavr-Savr番茄。最重要的商业转基因作物是玉米，大豆，棉花，油菜籽，土豆，烟草，南瓜，木瓜，菊苣。如今，几乎所有重要的作物都用于遗传地人工改性。用于识别国际运输中的GMO产品的方法应高效，敏感，节省时间和准确。 PCR广泛用于该领域。降低PCR敏感性和保证的关键因素是制备高纯度样品核酸和来自非特异性扩增分子的靶扩增产物的鉴别。今天没有任何方法，可以广泛用于制备来自所有最重要的植物的PCR核酸。开发了一种可靠的新方法，用于检测转基因科学博斯。该方法可以广泛使用，并且是上述PCR限制因子的分辨率。

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