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首页> 外文会议>International Conference on Remediation of Chlorinated and Recalcitrant Compounds >High-Throughput Sequencing as a Tool to Monitor Microbial Community Evolution in a Tetrachloroethene-Contaminated EPA Superfund Site(PPT)
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High-Throughput Sequencing as a Tool to Monitor Microbial Community Evolution in a Tetrachloroethene-Contaminated EPA Superfund Site(PPT)

机译：高通量测序作为监测四氯乙烯污染的EPA超级朋格（PPT）中微生物群落演变的工具

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摘要

High-throughput sequencing (HTS) techniques are revolutionizing biomedical sciences and have the same potential for environmental sciences. A proof-of-concept pilot project was undertaken to determine the efficacy of HTS to monitor the evolution of a microbial community in a tetracholorethene-contaminated aquifer undergoing remediation by enhanced reductive dechlorination (ERD). The selected groundwater remedy underway at the North Railroad Avenue Plume (NRAP) Superfund Site in Espa?ola New, Mexico (NPL #NMD986670156) is ERD using carbon source and nutrient addition. This aquifer is the sole source of potable water for Espa?ola and the adjacent Santa Clara Pueblo, but contamination from the NRAP source, a commercial drycleaner, forced the closure of two water supply wells. Prior to remedialaction, the NRAP groundwater plume comprised an approximate 58-acre, 260-foot-deep plume of tetrachloroethene, which is classified as a possible carcinogen.
机译：高通量测序（HTS）技术正在彻底改变生物医学科学，具有相同的环境科学潜力。求概念验证试验项目，以确定HTS以通过增强的还原脱氯（ERD）在进行修复的四硫代乙烯污染含水层中监测微生物群落的演变的疗效。在ESPA的北铁路大街羽流（NRAP）超级朋格上进行的所选地下水补救措施在eSPA？OLA New，墨西哥（NPL＃NMD986670156）是使用碳源和营养添加的ERD。这种含水层是ESPA饮用水的唯一来源？OLA和邻近的SANTA CLARA PUEBLO，但来自NRAP源的污染，商用干CHERENER，迫使两个供水井。在重新统计之前，NRAP地下水羽形包括大约58英亩，260英尺深的四氯乙烯，其被分类为可能的致癌物质。

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