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首页> 外文会议>International Conference on Remediation of Chlorinated and Recalcitrant Compounds >Next Generation qPCR: High-Throughput, Highly Parallel qPCR Arrays (QuantArrays) for Comprehensive Site Assessment
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Next Generation qPCR: High-Throughput, Highly Parallel qPCR Arrays (QuantArrays) for Comprehensive Site Assessment

机译：下一代QPCR：高吞吐量，高度平行的QPCR阵列（QuantraRsays），用于综合网站评估

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摘要

Background/Objectives. Quantitative polymerase chain reaction (qPCR) is a robust, commonly applied environmental molecular diagnostic (EMD) to quantify gene targets in sediment and groundwater. Dehalococcoides mccartyi (Dhc) quantification has become an indispensable component of assessment, remedy selection, and performance monitoring at chlorinated ethene sites. Standard qPCR quantification, of a single gene target at a time, provides valuable but limited information on the microbial community involved in bioremediation processes. Specific reductase genes required for vinyl chloride and chloroform reductive dechlorination are not universally distributed among known halorespiring strains. Other organohalide respiring bacteria are directly responsible for biodegradation of common co-contaminants like chlorinated ethanes, while an array of microbial groups play supporting or antagonistic roles. Depending upon subsurface conditions, aerobic or cometabolic mechanisms may dictate contaminant destruction. This presentation builds upon the Interstate Technology & Regulatory (ITRC) EMD team’s qPCR guidance documents, and describes newly identified functional genes and the development of high-throughput qPCR arrays (QuantArrays) to obtain more comprehensive quantification of microbial populations critical for site remediation.
机译：背景/目标。定量聚合酶链反应（qPCR的）是健壮的，普遍适用的环境的分子诊断（EMD）来量化基因在沉积物和地下水的目标。 Dehalococcoides mccartyi（DHC）量化已成为评估，补救措施的选择，以及性能的氯化乙烯网站监控不可或缺的组成部分。标准定量PCR定量，同时单个基因的目标，提供了涉及生物修复过程中的微生物群落宝贵的，但有限的信息。氯乙烯和氯仿还原脱氯需要特定还原酶基因并不普遍已知halorespiring菌株之间分配。其他有机卤化物呼吸细菌是用于普通共污染物，如氯化乙烷的生物降解直接负责，而微生物组的阵列起支承或拮抗作用。取决于地下条件，需氧或共代谢机制可支配污染物破坏。此演示文稿建立在州际技术与管理（ITRC）EMD球队的qPCR的指导性文件，并介绍新发现的功能基因和高通量定量PCR阵列（QuantArrays）的发展，获得微生物种群的场地修复关键的更全面的定量。

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