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Evidence of gains in nutritional quality in Lathyrus sativus

机译：Lathyrus sativus营养质量收益的证据

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By exploiting somaclonal variation a large number of stable low ODAP containing L. sativus lines have been developed. Some of the low ODAP strains also have high yields. One such line, Bio L212 has been released. A few of the somaclones have been DNAfinger printed. An ODAP degrading gene has been isolated and characterised from a soil microbe and has been cloned into an Agrobacterium strain for transforming L. sativus. Lathyrus sativus, grass pea or Khesari is an important grain legume of India, Bangladesh, Nepal and Ethiopia. It is cultivated over an area of about 1.5 million ha with the annual production of about 0.8 millions tonnes in India. In Bangladesh it is the most important legume crop. It is a hardy crop and is resistant to adverse conditions of drought and water logging and requires minimum inputs for its cultivation. Being a grain legume it also enriches the soil. It has a protein content varying between 28-34%. But the full potential of this crop could not be exploited because of thedevelopment of neurolathyrism characterised by lower limb paralysis in people who consume the grain over a prolonged period as a staple of their diet. The causative agent for neurolathyrism has been identified as- (N-oxalyl) amino alanine (BOAA) or N-oxalyl) -2,3-diaminopropionic acid (ODAP) by Sarma and Padmanaban (1969). Malathi et al (1967, 1970) proposed a two step reaction for the synthesis of ODAP Oxalyl CoA Synthetase 1. Oxalate + CoA+ATP=Oxalyl-CoA+AMP+Pi. ODAP Synthase 2. Oxalyl CoA+DAP=ODAP+CoA It has been reported that irrespective of varieties and stages of development, ODAP is detected in all tissues of Lathyrus plants but maximum content was observed in leaves during the vegetative stage and in the embryo during the reproductive stage(Prakash et al 1977). Despite a ban on its sale, Lathyrus is grown along with other rabi crops to provide an alternative crop during times of adverse conditions. In the eastern part of India it is extensively grown as "Para" or "Utera" with rice, by broadcasting Lathyrus seeds in rice fields about 2-4 weeks before the rice harvest. This practice not only minimizes the work but helps to harvest a second crop without any input.
机译：通过利用索华级别，已经开发了大量稳定的低通孔，其含有L. Sativus线。一些低常规菌株也具有高收益率。一条这样的线路，BIO L212已被释放。少数糖蜜是Dnafinger印花。已经分离出常规降解基因并从土壤微生物中分离并克隆到用于转化L.Sativus的土壤杆菌菌株中。 Lathyrus sativus，草豌豆或瓜氏素是印度，孟加拉国，尼泊尔和埃塞俄比亚的重要谷物。它的培育范围约为150万公顷，在印度的年产量约为0.8万吨。在孟加拉国，这是最重要的豆科植物作物。它是一种耐寒性的作物，抵抗干旱和水测井的不利条件，需要培养的最低输入。作为谷物豆类，它也丰富了土壤。它的蛋白质含量在28-34％之间变化。但是由于神经肠道的开发，这种作物的全部潜力不能被剥削，这些作物在长期的人中消耗谷物的人们少肢体瘫痪作为其饮食的主食。通过Sarma和Padmanaban（1969）鉴定了神经旋涡术的致原因是鉴定为 - （n-氧基氧基）氨基丙氨酸（BOAA）或N-氧基亚氨基甲酸（ODAP）（1969）。 Malathi等人（1967,1970）提出了两步反应，用于合成Odap Oxalyl CoA合成酶1.草酸盐+ CoA + ATP = Oxalyl-CoA + Amp + Pi。 Odap合酶2. oxalyl coa + dap = odap + coa据报道，与发育的品种和阶段无关，在植物植物的所有组织中检测到ODAP，但在植物阶段和胚胎中观察到最大含量。生殖阶段（Prakash等人1977）。尽管禁止其销售，但Lathyrus与其他Rabi作物一起成长，以在不利条件下提供替代作物。在印度的东部，通过在大米收获前2-4周的大米领域的Lathyrus种子广播Lathyrus籽，广泛地长大地长大为“帕拉”或“子宫”。这种做法不仅最大限度地减少了工作，而且有助于收获第二种作物，而无需任何输入。

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