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Sequence analysis and expression of human papillomavirus 16 late gene L1 obtained from cervical carcinoma tissues

机译:从宫颈癌组织中获得的人乳头瘤病毒16晚期基因L1的序列分析及表达

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摘要

Human papillomavirus type 16(HPV16) is highly related with the development of cervical carcinoma. HPV has two late genes:L1 and L2. L1 codes the main capsid protein. With two specific primers, the open reading frames (ORF) of HPV 16 L1 gene were amplified and cloned from three cervical carcinoma tissues samples obtained from Chinese patients by PCR and TA cloning strategy. The sequences of the cloned L1 gene had been analyzed.
机译:人乳头瘤病毒16型(HPV16)与宫颈癌的发育具有高度相关。 HPV有两个晚期:L1和L2。 L1编码主衣壳蛋白。通过两个特异性引物,扩增HPV 16 L1基因的开放阅读框(ORF)并从PCR和TA克隆策略从中国患者获得的三种宫颈癌组织样本中克隆。分析了克隆的L1基因的序列。

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