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首页> 外文会议>International conference on arsenic exposure and health effects >Arsenic Exposure, Null Genotypes of Glutathione S-Transferase M1,T1 and P1, and Risk of Carotid Atherosclerosis among Residents in the Lanyang Basin of Taiwan
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Arsenic Exposure, Null Genotypes of Glutathione S-Transferase M1,T1 and P1, and Risk of Carotid Atherosclerosis among Residents in the Lanyang Basin of Taiwan

机译：砷曝光，谷胱甘肽S-转移酶M1，T1和P1的零基因型，以及台湾兰阳盆地居民患者颈动脉粥样硬化的风险

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In order to evaluate the synergistic effects of arsenic exposure through drinking water and genetic polymorphisms of GST M1, T1, and P1 on the risk of carotid atherosclerosis, a total of 605 residents who participated physical examination of arsenic-induced health effects held in Lanyang Basin were recruited as study subjects. A standardized personal interview based on a structured questionnaire was carried out to obtain information on duration of well water consumption, so-ciodemographic characteristics, cigarette smoking, alcohol drinking, as well as personal and family history of hypertension, diabetes and cerebrovascular disease. Well water samples were collected during the home interview. Hydride generator combined with flame atomic absorption spectro-metry was used to determine the arsenic concentration in these samples. Whole blood samples were collected and centrifuged to separate buffy coat. DNA was then purified from buffy coat and stored at -20°C until genotyping. PCR was used for genotyping GSTM1 and GSTT1. PCR-RFLP was also carried out to determine the genetic polymorphisms of GST P1. Intimal-medial thickness (IMT) of arterial walls was measured by B-mode real-time ultrasound as an index of atherosclerosis. Plaque score was also used as an index of atherosclerosis. Logistic regression analysis was used to estimate age-sex-adjusted odds ratio and 95% confidence interval. Age was a major risk for the development of carotid atherosclerosis, showing a significant dose-response relationship. Compared with the youngest age group, the significant odds ratios of 5.9 and 2.3 were observed for the oldest and middle age groups, respectively. Significant risk for occurrence of carotid atherosclerosis was also observed among hypertension patients with age-sex-adjusted odds ratio of 2.4. Significant age-sex-adjusted odds ratios of risk of carotid atherosclerosis were observed in exposure groups with arsenic concentration in well water greater than 50 μg/l, showing odds ratio of 2.5. Moreover, study subjects with cumulative arsenic exposure greater than 1.0 mg/l-year also had significant age-sex-adjusted risk of developing carotid atherosclerosis. A significant higher age-sex-adjusted odds ratio of 2.0 for the development of carotid atherosclerosis was observed among study subjects with genotypes of W/M and M/M for GSTP1. A significant dose-response relationship was observed between risk of carotid atherosclerosis and arsenic exposure indices of arsenic concentration in well water and cumulative arsenic exposure among study subjects with null genotype of GSTM1, T1 and with W/M or M/M genotype of GSTP1. In conclusion, a significant effect on the risk of carotid atherosclerosis was observed among study subjects with arsenic exposure and with genotypes of W/M and M/M. In addition, significant synergistic effects on the risk for development of carotid atherosclerosis were also found between arsenic exposure and various genotypes of GST M1.
机译：为了通过对颈动脉粥样硬化的危险性饮酒GST M1，T1和P1的水和遗传多态性，以评估砷暴露的协同效应，共605个居民谁参加了兰阳盆地举行的砷中毒引起的健康影响体检被招募为研究对象。基于结构式问卷的标准化面试进行到获得好水的消耗，所以，ciodemographic特点，吸烟，饮酒，以及高血压，糖尿病，脑血管疾病的个人史，家族史的持续时间的信息。在入户访谈期间收集井的水样。氢化物发生器火焰原子吸收分光metry组合用于测定砷浓度这些样品英寸全血样品收集和离心以分离血沉棕黄层。 DNA然后从血沉棕黄层纯化并保存在-20℃直至基因分型。 PCR技术用于基因分型GSTM1和GSTT1。 PCR-RFLP也进行了以确定GST P1的遗传多态性。动脉壁的内膜 - 中层厚度（IMT）通过B模式实时超声测量为动脉粥样硬化的指标。斑块分数也用作动脉粥样硬化的指标。逻辑回归分析来估计年龄性别校正的比值比和95％置信区间。年龄是动脉粥样硬化的发展的一个主要风险，呈现出显著的剂量 - 反应关系。与最年轻的年龄组相比，观察到的最古老的和中等年龄组的5.9和2.3的显著比值比，分别。对动脉粥样硬化的发生显著风险也高血压患者中观察到的2.4年龄和性别调整后的赔率比。在暴露组中观察到与砷浓度井水大于50微克/升动脉粥样硬化的风险的显著年龄性别校正的比值比，显示出2.5的比值比。此外，研究对象与累积砷暴露大于1.0毫克/升，一年也有发展动脉粥样硬化的显著年龄性别调整风险。研究受试者与W / M和M / M为GSTP1的基因型中观察到的2.0颈动脉动脉粥样硬化的发展甲显著更高年龄性别校正的比值比。颈动脉动脉粥样硬化和在井水和累积砷暴露研究受试者与GSTM1，T1的空白基因型和与W / M或GSTP1的M / M基因型中砷浓度的砷曝光指数的风险之间观察到显著的剂量 - 反应关系。总之，在人群当中的研究对象与砷暴露与W / M和M / M的基因型观察颈动脉粥样硬化的风险显著的效果。此外，砷暴露与GST M1的不同基因型之间也发现了对动脉粥样硬化的发展风险显著的协同效应。

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