Rice blast disease is widespread throughout Thai rice fields, especially in those planted to well-known cultivar KDML105. This disease is caused by an ascospore fungus, Pyricularia grisea (anamorph Magnaporthe grisea). Many methods have been applied in DNA fingerprinting of the Thai R grisea, including random amplified polymorphic DNA (RAPD). The RAPD pattern of some rice-host isolates had revealed diversity in different cultivars. The other method used to study fingerprinting pattern is AFLP (amplified fragment length polymorphism). A small-scale cluster based on this method differed from the cluster from the RAPD technique, However, this technique can be used to separate barley isolates from rice isolates. These selected methods were used in determining diversity in Thai P. grisea populations. The cluster characterized by its genetics will be compared with cluster data obtained by pathotyping.
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