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首页> 外文会议>Mexican Symposium on Medical Physics >Kinetic of the Intracellular Incorporation of New Phthalocyanines Synthesized in Mexico and Its Potential as Photosensibilizers in the Photodynamic Therapy
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Kinetic of the Intracellular Incorporation of New Phthalocyanines Synthesized in Mexico and Its Potential as Photosensibilizers in the Photodynamic Therapy

机译：墨西哥合成的新酞菁的细胞内掺入的动力学及其在光动力学疗法中的光敏剂潜力

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The search of more specific and efficient photosensitizer in low oxygen tensionsis a need in the Photodynamic Therapy (PDT). Phthalocyanines have demonstrated to havethe above mentioned activity. The aim of this work was to determine the efficiency of PDTusing two phthalocyanines synthesized in Mexico to eliminate melanoma cells. B16F0melanoma mouse cells were exposed to concentrations from 8.95x10~(-5)to 0.733 mg/mL ofF16VoPc and F 16NbPcC13 during 24h, afterwards cellular mortality was measured. Onekinetic was realized to determine the intracellular incorporation of phthalocyanines byconfocal microscopy at 1, 2, 4, 8, 16 and 24 h of exposition. The PDT was applied exposingthe cells to innocuous concentration (that does not provoke cellular death with outirradiation) and irradiating with an argon laser at 100 J/cm~2.For each phthalocyanine acontrol group was used; one group was not treated neither with light nor withphthalocyanine, the other group it was only irradiated. 24 h after treatment the citotoxicitywas measured by Alamar blue assay. The innocuous concentration found for thephthalocyanines F 1 6VoPc and F 16NbPcC13 were 4.58x10-2 and 2.29x10~(-2)mg/mL,respectively. The time of maximum intracellular accumulation for both phthalocyanines was24 h. Only the Fl6VoPc had anticancerous activity and induced 31.7 % of cellular death.The PDT might offer a potential alternative to the treatment of this cancer when is used thephthalocyanine F16VoPc.
机译：更具体，在低氧气高效光敏剂的搜索tensionsis在光动力疗法（PDT）的需求。酞菁已展示出havethe上述活动的上方。这项工作的目的是确定在PDTusing墨西哥合成消除黑色素瘤细胞2个酞菁的效率。 B16F0melanoma小鼠细胞暴露于浓度为8.95x10〜（-5），以0.733毫克/毫升ofF16VoPc和F 16NbPcC13在24小时期间，之后细胞死亡测定。 Onekinetic被实现以确定在论述的1，2，4，8，16和24小时酞菁byconfocal显微镜的细胞内掺入。该PDT应用于exposingthe细胞无害的浓度（不挑起与outirradiation细胞死亡）并在100焦耳/平方厘米〜2.对于每个酞菁，使用acontrol组氩激光照射;一个基团不与光也不withphthalocyanine，它仅照射的另一组既不处理。治疗后24个小时，通过Alamar蓝测定法测量的citotoxicitywas。找到thephthalocyanines f 1的6VoPc和F 16NbPcC13无害浓度分别为4.58x10-2和2.29x10〜（-2）毫克/毫升。两个酞菁was24 h的最高细胞内积累的时间。只有Fl6VoPc有抗癌活性和细胞还有死亡。PDT诱发的31.7％在使用thephthalocyanine F16VoPc可能提供这种癌症治疗的潜在替代品。

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