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首页> 外文会议>International In Situ and On-Site Bioremediation Symposium >ASSESSMENT OF PETROLEUM BIODEGRADATION POTENTIAL WITH ALTERNATIVE ELECTRON ACCEPTORS
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ASSESSMENT OF PETROLEUM BIODEGRADATION POTENTIAL WITH ALTERNATIVE ELECTRON ACCEPTORS

机译：用替代电子受体评估石油生物降解潜力

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A laboratory scale experiment was performed to test the biodegradation potential of petroleum compounds under aerobic, Fe(Ⅲ)-reducing and nitrate-reducing conditions. Serum bottles were prepared with 100 mL of nutrient solution, 1 g of sediments from the San Jacinto Wetland Research Facility (SJWRF) as a seed and chemically dispersed crude oil. The Fe(Ⅲ) treatment received 518 mg/L of amorphous iron while the nitrate-reducing treatment received 390 mg/L of KNO_3. The aerobic treatment was started with a laboratory air headspace and received pure oxygen additions every two days. Biodegradation was observed under all conditions for both n-alkanes from n-C_(10) through n-C_(35) and polynuclear aromatic hydrocarbons (PAHs) with four rings and less. Average first-order decay coefficients for n-C_(16) to n-C_(20) were 0.114, 0.307 and 0.712 (day~(-1)) for the Fe(Ⅲ)-reducing, aerobic and nitrate-reducing treatments, respectively. Phenanthrene first-order decays were 0.097, 0.122 and 0.346 (day~(-1)) for the treatments in the same order. Based on this experiment, the bacterial population at SJWRF is capable of degrading the various petroleum compounds studied under aerobic, NO_3-reducing and Fe(Ⅲ)-reducing conditions.
机译：进行了实验室规模的试验，以测试石油化合物的生物降解潜力在需氧，铁（Ⅲ）还原和硝酸盐还原条件。血清瓶用100ml营养液，1g的从圣哈辛托湿地研究基金（SJWRF）作为种子沉积物和化学分散原油制备。而硝酸盐还原处理接收KNO_3的390毫克/升铁（Ⅲ）处理接收到的无定形铁的518毫克/升。好氧处理开始用实验室空气顶空和接受纯氧补充每两天。生物降解是为两个正构烷烃的所有条件下观察到从正C_（10）通过正C_（35）和多核芳香烃（PAHs）具有四个环和更小。对于n-C_（16）平均一阶衰减系数以正C_（20）分别为0.114，0.307和0.712（天〜（-1））的铁（Ⅲ）还原，需氧和硝酸盐还原处理，分别。菲一阶衰变为0.097，0.122和0.346在同一顺序的处理（日〜（-1））。基于该实验中，在SJWRF细菌群体是能够降解有氧，NO_3还原和Fe（Ⅲ）还原的条件下研究了各种石油化合物。

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