首页> 外文会议>International symposium of the ICP-BR Bee Protection Group >A bi-tunnel method developed to investigate the side-effects of systemic seed dressings or systemic soil treatments on honeybees, Apis mellifera
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A bi-tunnel method developed to investigate the side-effects of systemic seed dressings or systemic soil treatments on honeybees, Apis mellifera

机译:开发的双隧道方法,以研究全身种子敷料或全身土壤治疗对蜜蜂,Apis Mellifera的副作用

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A semi-field method was developed to assess the side-effects of systemic seed dressings or systemic soil treatments with plant protection products on honeybees (Apis mellifera), which allows a direct pre- and post-treatment comparison of the effects. The proposed methodology takes also into account the requirements outlined in the EPPO 170, BBA VI,23-1 and C.E.B. 129 guidelines. The study design is based on the release of bees in a bi-tunnel system: two adjacent tunnels, each 20 m X 8 m wide and aheight of 3.5 m at the highest point, covered with a white fine mesh plastic netting. Three replicated bi-tunnels per treatment and the control are set up. In all the control plots, both tunnels of each replicate are untreated and therefore planted onlywith control plants, while in the test item replicates one tunnel is untreated (i.e. planted with control plants) and the second tunnel is treated (e.g. dressed seeds, treated soil). In order to prolong the exposure time of the bees (flowering plants available over a longer period), the crop planting is staggered. Bee colonies headed by sister queens with a size of approximately 10000-15000 bees will be set up at one end of the bi-tunnel between the 2 tunnels. Each hive is provided with a pollen trap, adead bee trap and Apiscan. A removable netting between the two tunnels allows to control in which tunnel(s) the bees will be able to forage. The complete study consists of 3 successive periods of exposure (each exposure 5-7 days) of the bees: 1) bees will be allowed to invade the untreated half of the bi-tunnel and the behavior of each individual colony will be assessed, 2) the netting separating the 2 tunnels will be removed and the bees will be allowed to freely invade the treated and untreated areas(dynamics of invasion and the behavior of colonies will be assessed in order to study potential repellent effects) and 3) the bees will be confined in the second tunnel (treated tunnel) and behavior of the colonies observed.
机译:开发了一种半场方法,以评估全身种子敷料或全身土壤处理的副作用,植物保护产品对蜜蜂(Apis Mellifera),这允许直接预先和治疗后的效果比较。所提出的方法还考虑了EPPO 170,BBA VI,23-1和C.B中概述的要求。 129指南。该研究设计基于双隧道系统中的蜜蜂的释放:两个相邻的隧道,每扇形,每20米x 8米宽,Aheight在最高点处为3.5米,覆盖着白色的细网塑料网。设置三个复制的双隧道,并设置控制。在所有控制图中,每种复制的两个隧道都未处理,因此仅种植控制植物,而在测试项目中,重复一条隧道未经处理(即用对照植物种植),并治疗第二隧道(例如穿着种子,处理的土壤) )。为了延长蜜蜂的暴露时间(在较长时间可用的开花植物),作物种植被交错。在2个隧道之间的双隧道的一端,由尺寸约为10000-15000蜜蜂的姐姐座的蜜蜂殖民地将在2个隧道之间的一端设置。每个蜂巢都设有花粉陷阱,Adead Bee陷阱和Apiscan。两个隧道之间的可拆卸网允许控制哪些隧道蜜蜂能够觅食。完整的研究包括3蜜蜂的连续曝光(每次暴露5-7天):1)蜜蜂将被允许入侵双隧道的未经处理的一半,每个殖民地的行为将被评估2,2 )将移除2个隧道的网将被移除,并且蜜蜂将被允许自由地侵入治疗和未处理的区域(侵袭的动态以及菌落的行为将被评估,以便研究潜在的驱蚊效应)和3)蜜蜂将被限制在第二隧道(处理过的隧道)和观察到的菌落的行为。

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