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首页> 外文会议>International Congress of the Acarology >Bioassay, radiobiosynthesis, and GC/MS analysis of juvenile hormone in ticks: a new perspective
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Bioassay, radiobiosynthesis, and GC/MS analysis of juvenile hormone in ticks: a new perspective

机译：蜱虫中幼年激素的生物测定，radiobios合成和GC / MS分析：一种新的视角

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Synthesis of the insect juvenile hormone (JH) homologues and their precursors and the measurement of JH titer were investigated in two tick species, Dermaceritor variables and Ornithodoros parkeri. No JHI, JHII, JHIII, JHIII bisepoxide, methyl famesoate or famesol was synthesised in vitro from [methyl-~3H]-methionine and famesoic acid or from [I-~(14)C]-acetate by synganglion, salivary gland, gut, ovary, fat body or muscle from multiple developmental stages and/or in larvae and adults. The same results were obtained with backless ticks incubated with JH precursors in vitro and when [methyl-~3H]-methionine was injected into ticks. Diploptera punctota, Gromphadorhina portentosa or Periplaneta americana were used as positive controls in these experiments and produced farnesol, methyl famesoate and/or JHIII. No juvenilising activity was found in lipid extracts from eggs, larvae, nymphs or adults of D. variabilis using the Galleria pupal cuticle bioassay. Lipid extracts from D. puncrato and lOOpg of JHIII were positive controls in these experiments. No JHI, JHII, JHIII, JHIII bisepoxide, methyl farnesoate or famesol was detected in hemolymph extracts from blood fed, virgin and replete D. variabilis or blood fed 0. parkeri females analyzed by selective ion, electron impact, gas chromatograpy-mass spectrometry (El GC-MS). The same technique was used for the accurate measurement of JH from hemolymph of D. punctata as a positive control. In our investigations of D. variabilis and/or D. parkeri, we detected no synthesis of the common insect juvenile hormones or precursors, none of the aforementioned juvenoids in tick hemolymph by El GC-MS, nor juvenilising activity associated with tick extracts using the Galleria bioassay.
机译：昆虫保幼激素（JH）同源物和它们的前体和JH滴度的测量的合成两种蜱物种，Dermaceritor变量和钝缘蜱parkeri进行了调查。没有JHI，JHII，JHIII，JHIII双环氧化物，甲基famesoate或法呢醇是在体外合成从[甲基 - 〜3H] - 甲硫氨酸和famesoic酸或由[I-〜（14）C]通过集合神经节 - 乙酸甲酯，唾液腺，肠，卵巢，脂肪体或肌肉从多个发展阶段和/或幼虫和成虫。相同的结果用无靠背蜱与体外JH前体和时温育获得的[甲基 - 〜3H] - 甲硫氨酸注入蜱。 Diploptera punctota，Gromphadorhina portentosa或美洲大蠊用作在这些实验中的阳性对照和产生法呢醇，甲基famesoate和/或JHIII。没有juvenilising活动在从卵，幼虫，若虫或D的成人脂肪提取物中发现使用的Galleria蛹表皮生物测定变异。从D puncrato和JHIII的lOOpg脂提取物是在这些实验中的阳性对照。没有JHI，JHII，JHIII，JHIII双环氧化物，在从血液中血淋巴提取物中检测金合欢酸甲酯或法呢醇进料，处女和充满D.变异或血液供给0. parkeri雌性分析通过选择性离子，电子冲击，气体色谱法 - 质谱法（埃尔GC-MS）。用于JH的从点状D.血淋巴作为阳性对照的精确测量相同的技术。在我们的D的调查变异和/或D. parkeri，我们没有检测到合成使用所述蜱提取物相关的常见的昆虫保幼激素或前体，没有通过埃尔GC-MS在剔血淋巴上述保幼激素类似物，也不juvenilising活性广场生物测定。

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