• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Congress of the Acarology >Tick immunity to microbial infections: control of representative bacteria in the hard tick Dermacentor variabilis (Acari: Ixodidae)
【24h】

Tick immunity to microbial infections: control of representative bacteria in the hard tick Dermacentor variabilis (Acari: Ixodidae)

机译：蜱免疫性对微生物感染：控制硬蜱皮特氏菌的代表性细菌（Acari：Ixodidae）

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fed female ticks, Dermacentor vanabilis, survived for at least 5 days without significant mortality following intrahemocoelic inoculation of the bactenal species Bacillus subtilis, Staphytocaccus aureus, Micmcoccus luteus, Escherichia coll, and Borrelio burgdorferi. In contrast, survival of ticks 5 days after inoculation with Pseudomonas aeruginosa was only 12 percent and the few surviving individuals appeared moribund. However, survival of ticks inoculated with heat-killed P. aerurginosa was 100%,suggesting that lethality of this bacterial species required viable cells. D. vanabilis was found to control E. coli, a representative gram negative bacterium, very rapidly. Some control was evident in as little as 1 hour after bacterial inoculation, and all E. coli cells were cleared from the hemo-lymph within 6 hours. This is a much more rapid response than that previously observed for B. subtilis. D. vanabilis hemolymph plasma from E. coli-challenged ticks inhibited microbial growth, but hemolymph from non-challenged ticks did not inhibit growth of E. coli. This differs from studies with ticks challenged with B. sufatife, which were found to be constitutively anti-microbial. Anti-E. coli activity of hemolymph plasma from challenged ticks was correlated with two relatively non-polar fractions separated by HPLC using a reversed phase Vydac (C_4) protein column. However, these fractions had no effect on 6. subtife. Similarly, fractions separated by HPLC from B. subtilis-challenged ticks had no effecton E. coli growth. In addition to its activity against the non-vectored, non-pathogenic bacteria, D. variabils hemolymph from E. coli-stimulated ticks was also found to be borreliocidal against the Lyme disease spirochete, Boirelia burgdorferi. Furtherstudy is needed to purify and identify the antimicrobial proteins or peptides active in tick hemolymph against this array of different types of microbes.
机译：美联储女性蜱虫，无皮佐尔·瓦斯巴里斯，在没有显着死亡的情况下幸存下来至少5天，在内部胚胎物种芽孢杆菌枯草芽孢杆菌，Staphytocaccus金黄色葡萄球菌，麦克芹菌叶黄素，大肠杆菌和Borrelio Burgdorferi。相比之下，与假单胞菌铜绿假单胞菌接种后蜱的存活仅为12％，少数幸存的人出现了奄奄一息。然而，接种用热杀死的P. AeruginoSA接种的蜱的存活率为100％，表明这种细菌物种的致死性需要活细胞。 D. Vanabilis被发现控制大肠杆菌，代表性革兰氏阴性细菌，非常迅速。在细菌接种后，一些控制在短至1小时内显而易见，并且在6小时内从血液淋巴中清除所有大肠杆菌细胞。这是比以前观察到的B.枯草芽孢杆菌的快速响应。 D.来自大肠杆菌攻击性蜱的Vanabilis血淋巴血浆抑制微生物生长，但来自非攻击性蜱的血淋巴不抑制大肠杆菌的生长。这与用B. Sufatife挑战挑战的蜱虫的研究不同，发现该研究组成抗微生物。反e。来自攻击蜱的血淋巴血浆的COLI活性与使用反相Vydac（C_4）蛋白质柱的HPLC分离的两个相对非极性级分相关。然而，这些馏分对6例无效。类似地，由枯草芽孢杆菌攻击性蜱分离的HPLC分离的级分没有效应大肠杆菌生长。除了对非向量的非致病细菌的活性外，也发现来自大肠杆菌刺激的蜱的D.Variabils血淋巴。螺旋体，Boirelia Burgdorferi，Boirelia Burgdorferi也发现了来自大肠杆菌刺激的蜱的血淋巴。需要Furtherstudy来纯化和鉴定蜱血淋巴中活性的抗微生物蛋白或肽，而是针对这种不同类型的微生物阵列。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号