• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Congress on Photosynthesis >Investigation of bacteriochlorophyll biosynthesis genes in Blastochloris viridis by functional complementation of mutants of Rhodobacter capsulatus
【24h】

Investigation of bacteriochlorophyll biosynthesis genes in Blastochloris viridis by functional complementation of mutants of Rhodobacter capsulatus

机译：乳膜瘤突变体突变体突变体韧带血管生物合成基因的研究

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Blastochloris (formerly Rhodopseudomonas) viridis produces bacteriochlorophyll (BChl) b which is distinguished from BChl a by the possession of an ethylidene group at C8 instead of an ethyl (Eimhjellen et al., 1963). This chemical structure enablesto BChl b to absorb longer wavelength light (approx 1100 nm) than BChl a. To investigate genes involved in the BChl b biosynthesis, we have constructed genomic DNA libraries from B. viridis and introduced them to mutants of the nonsulfur purple bacteriumRhodobacter capsulatus which is deficient in BChl a biosynthesis. Total ten kinds of R. capsulatus mutants, three Mg-chelatase (BchD, H, I) mutants, three protochlorophyllide reductase (BchB, L, N) mutants, Mg-protoporphyrin IX methyltransferase (BchM)mutant, Mg-protoporphyrin IX monomethylester oxidative cyclase (BchE) mutant, bacteriochlorophyll synthase (BchG) mutant, and 3-vinylbacteriochlorophyllide hydratase (BchF) mutant, were functionally complemented by B. viridis library DNA. The complemented R. capsulatus strains were demonstrated to be capable of synthesizing BChl a under photoautotrophic growth conditions.
机译：Blastochloris（以前的Rhodopseudomonas）viridis产生的菌氯基（BCH1）B通过在C8代替乙基（Eimhjellen等，1963）中，通过BCHL A与BCHL A区分开（Eimhjellen等，1963）。这种化学结构EnableSto BCHL B吸收比BCHL A更长的波长光（大约1100nm）。为了探讨参与BCHL B生物合成的基因，我们已经构建了B.Viridis的基因组DNA文库，并将它们引入了非悬浮紫芽曲的突变体，其缺乏BCHL A生物合成。总共十种R. Capsulatus突变体，三种Mg-Chelatase（BCHD，H，I）突变体，三种蛋白氯化物还原酶（BCHB，L，N）突变体，Mg-原激霉素IX甲基转移酶（BCHM）突变体，Mg-原激霉素IX单甲基酯氧化环酶（BCHE）突变体，菌肤氯苯基合酶（BCHG）突变体和3-乙烯基苯二甲酸氯化物水合物（BCHF）突变体在功能上由B.Viridis库DNA互补。互补的R.Capsulatus菌株被证明能够在光营养植物生长条件下合成BCHL A.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号