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首页> 外文会议>International Congress on Photosynthesis >Supermolecular organization of Photosystem II and its associated lightharvesting antenna in the wild-type and npq4 mutant of Arabidopsis thaliana
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Supermolecular organization of Photosystem II and its associated lightharvesting antenna in the wild-type and npq4 mutant of Arabidopsis thaliana

机译：拟南芥野生型和NPQ4突变体中的光照组织的光照系统II及其相关的左氯化天线

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The thylakoid membranes of green plants build a highly complex and dynamic membrane system. Part of these membranes form the so-called grana stacks, which are enriched in PSII and LHCII. PSII-LHCII supercomplexes have been isolated by gentle detergent solubilization of spinach grana membranes or thylakoids, and their structures have been analysed by electron microscopy and image analysis of single particles at a resolution of about 20 A (see, e.g., Boekema et al., 1995, 1999ab; Nield et al., 2000a). The results revealed three types of binding sites of trimeric LHCII around a dimeric PSII core complex (designated S, M and L, from strongly, moderately and loosely-bound LHCII, respectively) and also three ways by which the PSII-LHCII supercomplexes are laterally associated to each other (to give the socalled megacomplexes). Analysis of semi-crystalline arrays in grana membranes revealed the supramolecular organization of the PSII-LHCII supercomplexes in the membranes (Boekema et al., 2000a).
机译：绿色植物的紫花状膜构建高度复杂和动态的膜系统。这些膜的一部分形成了所谓的甘蓝堆叠，富含Psii和Lhcii。 Psii-LHCII通过菠菜膜或囊体的温和洗涤剂溶解分离出PSII-LHCII超复杂性，并且它们的结构已经通过电子显微镜分析和以约20的分辨率的单颗粒的图像分析（参见，例如Boekema等， 1995,1999AB; Nield等，2000A）。结果揭示了三聚体LHCII的三种类型的三聚体LHCII的结合位点（分别从强烈，适度的，强烈，紧密结合的LHCII）以及三种方式，并且PSII-LHCII超复用横向彼此关联（给予考虑的Megacomplexes）。麦克纳膜中半晶阵列的分析显示膜中PSII-LHCII超复合体的超分子组织（Boekema等，2000A）。

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