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首页> 外文会议>International Congress on Photosynthesis >Thiol modulation of the chimeric ATP synthase complex of bacterial F_1 containing the regulatory region of the gamma subunit of chloroplast ATP synthase
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Thiol modulation of the chimeric ATP synthase complex of bacterial F_1 containing the regulatory region of the gamma subunit of chloroplast ATP synthase

机译：含有叶绿体ATP合酶γ亚基调节区的细菌F_1的嵌合ATP合酶络合物的硫醇调节

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The ATP synthase of chloroplast (CF_oCF_1) is activated by the proton gradient across the thylakoid membrane and modulated by the formation and the reduction of the disulfide bridge between two cysteine residues on the gamma subunit (thiol-modulation) (Nalin & McCarty 1984, Mills & Mitchell 1984). These two cysteines (~(199)Cys and ~(205)Cys, the numbers are for spinach chloroplast F_1-gamma) are located on the additional amino acid stretch region, 'regulatory region' (from ~(193)Ser to ~(241)Leu),of CF_1-gamma (Miki, et al. 1988). In the soluble CF1 reduction of the disulfide bond in gamma causes elicitation of the latent ATP hydrolyzing activity. To discover which residues in the region were important for this regulation, we studied the properties of the chimeric complex, which was prepared from the recombinant CF_1-gamma subunit and the recombinant TF_1-alpha and beta subunits (Hisabori, et al. 1997, 1998). The ATPase activity of this chimeric complex was regulated by the redox conditions aswe predicted. Then we prepared a mutant in the regulatory region of gamma subunit and investigated their regulatory function in the complex. From this study, we found key residues, the deletion of which reversed the regulatory function. The ATPase activity of this mutant complex was activated under oxidizing condition and inactivated under reducing conditions (Konno et al. 2000). In addition, we found that the region following the regulatory cysteines is important for the suppression of the enzymatic activity.
机译：叶绿体（CF_OCF_1）的ATP合成酶被质子梯度通过囊体膜激活并通过形成和两种半胱氨酸残基（硫醇调制）（Nalin＆McCarty 1984，Mills）的半胱氨酸残基之间的二硫桥的减少调节。 ＆mitchell 1984）。这两种半胱氨酸（〜（199）Cys和〜（205）Cys，这些数字用于菠菜叶绿体f_1-gamma）位于另外的氨基酸伸展区域，'调节区'（来自〜（193）Ser〜（ 241）leu），Cf_1-gamma（Miki，等，1988）。在可溶性CF1中，γ中二硫键的降低导致潜伏的ATP水解活性引发。为了发现该区域的哪些残留物对该调节很重要，我们研究了由重组CF_1-Gamma亚基和重组TF_1-α和β亚基制备的嵌合络合物的性质（Hisabori，等，1997,1997 ）。该嵌合络合物的ATPase活性由ASWE预测的氧化还原条件调节。然后我们在伽玛亚基的调节区制备了一个突变体，并在复杂中调查了他们的调节功能。从本研究开始，我们发现了关键残留物，删除监管职能的缺失。在氧化条件下激活该突变体复合物的ATP酶活性并在还原条件下灭活（Konno等，2000）。此外，我们发现调节性半胱氨酸后的区域对于抑制酶活性是重要的。

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