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Gene cloning and characterization of salt-inducible aldehyde oxidase in barley

机译：大麦盐诱导醛氧化酶的基因克隆与表征

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To study how plants respond and adapt to salt stress, we cloned hundreds of salt-inducible genes in barley (Hordeum vulgare L. cv. Haruna-nijyo) by differential displaymethod (Ueda et al, submitted). One of them is a cDNA clone (HvAO), encoding aldehyde oxidase (AO). AO (EC 1.2.3.1) has been extensively investigated in animals and microorganisms (Hall et al., 1986; Yoshihara et al., 1986). The enzyme has been implicated in the detoxification of various xenobiotics. In plants two AO catalyze the final step in biosynthesis of twophytohormones, through the oxidation of abscisic aldehyde to abscisic acid (ABA) (Walker-Simmons et al., 1989; Leydecker et al., 1995) and indole-3-acetaldehyde to indole-3-acetic acid (IAA) (Koshiba et al., 1996). Four Arabidopsis cDNAs were cloned andone of them encodes AAO3, which catalyzes the final step in the biosynthesis of ABA (Seo et al., 2000), whereas barley cDNAs have not been cloned and characterized yet. Three and four AO protein isoforms were detected in Arabidopsis (Seo et al., 2000) and barley (Omarov et al., 1999), respectively. ABA is synthesized by environmental stresses such as salt, drought and cold stress, and stomatal closure is triggered by ABA. To understand the relationship between HvAO and ABA synthesis, we studied the expression pattern of HvAO under various stresses.
机译：为了研究植物如何应对和适应盐胁迫，我们通过差异显示方法克隆大麦（Hordeum Vulgare L. CV）中的数百种盐诱导基因。（UEDA等人，提交）。其中一个是CDNA克隆（HVAO），编码醛氧化酶（AO）。 AO（EC 1.2.3.1）在动物和微生物中被广泛研究（Hall等，1986; Yoshihara等，1986）。酶已涉及各种异种症的排毒。在植物中，通过氧化脱离醛酸（ABA）氧化脱离醛酸（ABA）（1989; Leydecker等，1995）和吲哚-3-乙醛（Andole-3-乙醛）催化脱脂症的生物合成中的生物合成中的最终步骤-3-醋酸（IAA）（Koshiba等，1996）。克隆了四个拟南芥CDNA，它们中央可以编码AAO3，催化ABA的生物合成中的最后一步（SEO等，2000），而麦基CDNA尚未克隆和表征。在拟南芥（SEO等，2000）和大麦（Omarov等，1999）中检测到三和四种AO蛋白质同种型。 ABA由盐，干旱和冷应力等环境应力合成，并且由ABA引发气孔闭合。要了解HVAO与ABA合成的关系，我们研究了各种应力下HVAO的表达模式。

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