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首页> 外文会议>International Biohydrometallurgy Symposium >Chromosomal arsenic resistance genes from Sulfobacillus thermosulfidooxidans and a demonstration that the genetic diversity of arsB among the sulfobacilli is similar to that of their 16S rRNA genes
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Chromosomal arsenic resistance genes from Sulfobacillus thermosulfidooxidans and a demonstration that the genetic diversity of arsB among the sulfobacilli is similar to that of their 16S rRNA genes

机译：来自苏氟氟磺酰氧毒剂的染色体砷抗性基因和苏氟梭菌中ARSB遗传多样性的示范类似于其16S rRNA基因的遗传多样性

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Arsenic resistance genes were isolated from the moderately thermophilic, Gram-positive iron and sulfur-oxidizing bacterium, Sulfobacillus thermosulfidooxidans. Only arsR and arsB genes were present and attempts to identify an arsC using degenerate PCR primers or dependent arsC genes as probes in Southern hybridization experiments were unsuccessful. Although enhanced resistance to arsenite was not detected when the ars genes were cloned in Escherichia coli, the kumamolisin-As and arsRB genes were induced by arsenite. RT-PCR experiments suggested that transcription of the cloned kumamolisin-As-like and arsRB genes is linked in Escherichia coli, but not in Sb. thermosulfidooxidans. The gene order kumamolisin-As precursor, arsR and arsB was maintained among three strains of Sb. thermosulfidooxidans isolated from three continents. Southern hybridization using a Sb. thermosulfidooxidans arsB gene fragment as a probe gave a positive hybridization signal using S. acidophilus but not with S. thermotolerans genomic DNA. Comparison of partial sequence data of the arsB and 16S rRNA genes suggested that the two types of genes have undergone a similar evolutionary history and therefore that the arsB genes were present in the ancestral Sulfobacillus before its divergence into species.
机译：砷抗性基因与中等嗜热素，革兰氏阳性铁和硫氧化细菌分离，Sulfobacillus Thermosulfidooxidans。存在ARSR和ARSB基因并试图使用退化的PCR引物或依赖的ARSC基因鉴定ARSC作为南部杂交实验的探针是不成功的。虽然当在大肠杆菌中克隆ARS基因时，未检测到对亚砷酸盐的抗性，但是亚砷酸酯诱导kumamolisin-as和Arsrb基因。 RT-PCR实验表明，克隆的熊蛋白酶和ARSRB基因的转录在大肠杆菌中，但不在Sb中连接。热氟苯甲苯胺酰胺。基因令Kumamolisin - 作为前体，ARSR和ARSB在三种SB菌株中保持。热硫芬毒性血氧氮杂物从三大洲隔离。 Southern杂交使用SB。热硫醚氧酰胺氧化物作为探针的ARSB基因片段使用S.嗜酸纤维进行阳性杂交信号，但不含S.热溶剂素基因组DNA。 ARSB和16S rRNA基因的部分序列数据的比较表明，两种类型的基因经历了类似的进化史，因此在其分歧之前存在ARSB基因在其分解为物种之前存在于祖先苏术杆菌中。

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