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首页> 外文会议>International Biohydrometallurgy Symposium >Analysis of sulfur metasecretome in mixed cultures of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans
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Analysis of sulfur metasecretome in mixed cultures of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans

机译：酸酐氧嗪和酸酐硫氧化物硫含量硫含量分析

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The role of biomolecules in bioleaching of copper sulphide minerals carried out by bacterial consortia with predominating acidithiobacilli species is of outmost interest. The proteomic analysis on bioleaching bacterial strains have been focused up to date on full Acidithiobacillus ferrooxidans proteome, allowing the identification of proteins belonging to the general stress response, phosphate limiting conditions and the ones linked to the periplasmic fraction. Our study shows for the first time the differential expression of secreted proteins by means of standard proteomics between pure cultures of Acidithiobacillus thiooxidans and in mixture with A. ferroxidans (metasecretome) grown in sulfur, where a set of proteins is de novo synthesized in the mixed culture, identifying an Omp40-like protein possibly related to bacterial adhesion, an hypothetical protein PSEEN2944 with unknown function and up-regulation of a cytochrome c biogenesis protein, findings that give an insight into the role of proteins at the sulfur - bacteria interface, highlighting the outputs of bacterial interactions in biomining environments at the protein secretion level.
机译：生物分子在细菌结合中进行的硫化铜矿物质生物浸出的作用，其具有占优势的酸酐物种具有最小的感兴趣。生物浸出细菌菌株的蛋白质组学分析已经集中于全酸酐铁氧播尼蛋白质组，允许鉴定属于一般应力响应，磷酸盐限制条件和与周质级分相关的蛋白质。我们的研究表明，首次通过纯培养物与硫氧化物的纯培养物和混合物中的标准蛋白质组学表达分泌蛋白质的差异表达。在硫中生长的苯甲苯胺（Metasecretome），其中一组蛋白质是在混合中合成的德诺培养，鉴定与细菌粘附有关的OMP40样蛋白，一个假设的蛋白质Pseen2944，具有未知的功能和上调细胞色素C生物生成蛋白，结果表明介绍蛋白质在硫磺界面，突出显示蛋白质分泌水平的生物化环境中的细菌相互作用的产量。

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