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Adhesion of Acidithiobacillus caldus and Leptospirillum ferriphilum on pyrite and their effect on surface properties of sulfide minerals

机译:酸酐和Leptospirillum Ferriphilum对硫铁矿的粘附及其对硫化物矿物表面性质的影响

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Acidithiobacillus caldus and Leptospirillum ferriphilum cells grown in different energy substances (ferrous ion, sulfur and pyrite) were used. The adhesion of A. caldus and L. ferriphilum cells on pyrite and their effect on pyrite surface properties were studied by adsorption, zeta-potential and FT-IR methods, and the corrosion images of pyrite interaction with bacteria were examined using atomic force microscopy. Research showed that pyrite isoelectric point (IEP) after interaction with bacterial cells shifted towards cells isoelectric point, and the shift degree in case of interaction with A. caldus was observed to be much more pronounced than for interaction with L. ferriphilum, which can be due to higher affinity of A. caldus towards pyrite. The FT-IR spectra of pyrite treated with bacterial cells revealed the presence of the cell functional groups signifying cells adsorption. Although the adsorption density of A. caldus on pyrite was higher than that of L. ferriphilum, L. ferriphilum with strong ability to oxidize ferrous ion showed better leaching efficiency than A. caldus with strong ability to oxidize sulfur for pyrite leaching. The results demonstrated that more important of indirect action (L. ferriphilum) than direct action (A. caldus) on pyrite.
机译:使用在不同能量物质(亚铁离子,硫和硫铁粒)中生长的酸酐和瘦倍石的铁纤维细胞。通过吸附,ζ电位和FT-IR方法研究了A. Caldus和L.Ferriphilum细胞对硫铁矿的粘附性及其对硫铁矿表面性质的影响,并使用原子力显微镜检查硫铁矿与细菌的腐蚀图像。研究表明,在与细胞等电点移位的细菌细胞相互作用后的黄铁矿等电点(IEP),观察到与A. Caldus相互作用的换档程度比与L.Ferriphilum的相互作用更加明显,这可以是由于A. Caldus朝向黄铁矿的亲和力较高。用细菌细胞处理的黄铁矿的FT-IR光谱揭示了细胞官能团的存在,意味着细胞吸附。虽然硫铁矿上A的吸附密度高于L.Ferriphilum,L.Ferriphilum,L.Ferriphilum具有强大的氧化亚铁离子的能力,呈现出比A. Caldus氧化硫化硫化硫化物浸出的能力更好的浸出效率。结果表明,间接动作(L.Ferriphilum)比黄铁矿直接作用(A. Caldus)更重要。

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