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PLANT HORMONES: A KEY IN CLUBROOT DEVELOPMENT

机译:植物激素:马蹄莲开发的关键

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Clubroot disease causes gall formation on Brassica roots. A holistic approach was undertaken to understand the role of plant hormones in early clubroot symptom development in Arabidopsis. We used the CYCB1;1::GUS, DR5::GUS and ARR5::GUS constructs toassess clubroot initiation. We compared plant hormone concentration between control and infected plants, screened different hormone mutants for resistance and evaluated auxin transport. Also, a differential proteome study was performed using two dimensional gel electrophoresis coupled to MALDI-TOF. Combining the hormone and proteome data, we postulate that at the first stages of clubroot disease, cytokinins are produced by the pathogen. This triggers a local re-initiation of cell division in the root cortex. Consequently, a de novo meristematic area is established that acts as a sink for host-derived IAA, carbohydrates, nitrogen and energy to maintain the pathogen and to trigger gall development.
机译:甘蓝型疾病导致甘蓝根胆形成。采取了整体方法,了解植物激素在拟南芥早期的愈合症状发展中的作用。我们使用了cycb1; 1 :: gus,dr5 :: gus和Arr5 :: Gus构建Toassess Clubroot启动。我们比较了对照和受感染植物之间的植物激素浓度,筛选不同的激素突变体进行抗性和评估的养蛋白转运。此外,使用偶联至MALDI-TOF的二维凝胶电泳进行差异蛋白质组研究。结合激素和蛋白质组数据,我们假设在球杆菌疾病的第一阶段,细胞蛋白由病原体产生。这触发了根皮层中的局部重新启动细胞分裂。因此,建立了De Novo的共同型区域,其作为宿主衍生的IAA,碳水化合物,氮气和能量的沉没,以维持病原体和引发胆发育。

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