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Experiments on sexing yak spermatozoa by fluorescence in situ hybridisation using bovine Y-chromosome specific DNA probe

机译：使用牛y-染色体特异性DNA探针对原位杂交的荧光性牦牛精子的实验

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Y-bearing bovine spermatozoa may be distinguished by fluorescence in situ hybridisation (FISH) using specific DNA probe, offering an in vitro control of experiments for producing sex-oriented or sex-specific semen. Bovine Y-specific probe gave distinguishable signal also in yak spermatozoa. Specific binding of the probe to the corresponding sex chromosome was confirmed on yak chromosome preparations.

机译：α牛精子可以通过使用特异性DNA探针的原位杂交（鱼类）的荧光来区分，提供对生产性别或性别特异性精液的实验进行体外控制。牛y特异性探针在牦牛精子中也产生了可区分的信号。在Yak染色体制剂上证实了探针对相应的性染色体的特异性结合。

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《International Congress on Yak》|2002年||共4页
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作者
R. Tamas; Q. Xuebin; P. T. Erika; N. Szabolcs; H. Jianlin; K. Andras; T. Andras; S. Andras;
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正文语种
中图分类 S823.4-532;
关键词
cattle; fluorescence in situ hybridisation; sexing; spermatozoa; yak;

机译：牛;荧光原位杂交;性别;精子;牦牛;

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专利

1. Detection of cross-sex chimerism in the common marmoset monkey (Callithrix jacchus) in interphase cells using fluorescence in situ hybridisation probes specific for the marmoset X and Y chromosomes [J] . Wedi E., Mueller S., Neusser M., Reproduction, fertility, and development . 2017,第5期

机译：用荧光对Marmoset X和Y染色体的原位杂交探针对常见的细胞常见的细胞常见细胞交叉性斜/串的检测
2. Fluorescence in situ hybridization with Y chromosome-specific probe in decondensed bovine spermatozoa [J] . Kobayashi J, Kohsaka T, Sasada H, Theriogenology . 1999,第6期

机译：Y染色体特异探针在去浓缩牛精子中的荧光原位杂交
3. Diagnosis of bovine freemartinism by fluorescence in situ hybridization on interphase nuclei using a bovine Y chromosome-specific DNA probe [J] . Sohn SH, Cho EJ, Son WJ, Theriogenology . 2007,第7期

机译：利用牛Y染色体特异性DNA探针在相间核上进行荧光原位杂交诊断牛自由马丁
4. Experiments on sexing yak spermatozoa by fluorescence in situ hybridisation using bovine Y-chromosome specific DNA probe [C] . R. Tamas, Q. Xuebin, P. T. Erika, International Congress on Yak . 2002

机译：使用牛y-染色体特异性DNA探针对原位杂交的荧光性牦牛精子的实验
5. Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa. [D] . Delgado Callisaya, Pedro Angel. 2008

机译：储存过程中影响牛精子存活的因素，与含X染色体和Y染色体的精子的比例有关。
6. Molecular cytogenetic analysis of the crucian carp Carassius carassius (Linnaeus 1758) (Teleostei Cyprinidae) using chromosome staining and fluorescence in situ hybridisation with rDNA probes [O] . Aneta Spoz, Alicja Boron, Katarzyna Porycka, 2014

机译：利用染色体染色和rDNA探针的荧光原位杂交技术对fluorescenceCar（Linnaeus1758）（TeleosteiCyprinidae）进行分子细胞遗传学分析
7. Fluorescence in situ hybridization in diluted and flow cytometrically sorted boar spermatozoa using specific DNA direct probes labelled by nick translation [O] . I Parrilla, JM Vazquez, M Oliver-Bonet, 2003

机译：使用特异性DNA直接探针稀释和流动细胞血型分类野猪精子的荧光在稀释和流动细胞血上分类的野猪精子。

Experiments on sexing yak spermatozoa by fluorescence in situ hybridisation using bovine Y-chromosome specific DNA probe

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