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首页> 外文会议>International Congress on Yak >Efficient production of transgenic bovine/cat by microinjection and cloning technology of early embryos
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Efficient production of transgenic bovine/cat by microinjection and cloning technology of early embryos

机译：通过早期胚胎的显微注射和克隆技术有效地生产转基因牛/猫

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The present results suggested the feasibility of enhanced green fluorescent protein (EGFP) gene for non-invasive selection of transgenic bovine embryos at the pre-implantation stage by using the fluorescent microscope and linking the marker gene contacted with a desired gene. However, these injected embryos showed impaired development and high rate of the mosaic expression. Therefore, we conducted a trial to transfer the EGFP gene fragment into the bovine or cat foetus fibroblasts using polybrene for expressing bright green fluorescence in the whole inner cell mass. EGFP has a great advantage as a marker because the transgenic cells or organ can be observed at any time in their viable and intact state and it can be easily connected with pharmaceutical protein in vitro. Additional advantages of nuclear transfer combined with transgenesis become obvious when it is compared with microinjection techniques. In the future, we can produce the pharmaceutical proteins from cow or cat using nuclear transfer combining with the EGFP gene.
机译：本结果表明，通过使用荧光显微镜并将标记基因与所需基因接触的标记基因连接在预注入阶段，增强绿色荧光蛋白（EGFP）基因的可行性是在预注入阶段的非侵入性选择转基因牛胚。然而，这些注入的胚胎显示出显影性损害，使马赛克表达的高率高。因此，我们使用含有聚酶将EGFP基因片段转移到牛或猫胎儿成纤维细胞中的试验，以表达整个内部细胞质量的亮绿色荧光。 EGFP作为标记具有很大的优势，因为可以在其可行和完整状态下随时观察转基因细胞或器官，并且可以容易地与体外药物蛋白质连接。与显微注射技术相比，核转移与转基因联合转基因的额外优点变得明显。未来，我们可以使用与EGFP基因组合的核转移来生产来自牛或猫的药物蛋白。

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