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Studies on the biocompatibility of artificial organs and tissue engineered products: embryonic neuronal cell differentiation on the various kinds of biodegradable polymers

机译:人工器官和组织工程产品的生物相容性研究:各种可生物降解聚合物对胚胎神经元细胞分化

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Representative biodegradable-polymers are assayed to clarify the property when used as the materials for the nerve regeneration using the midbrain cell differentiation systems. As for polyglycolic acid [PGA], both the proliferation and differentiation of the midbrain cells were dose-dependently inhibited by PGA3000(M_w=3000). Poly(L-lactic acid)[PLLA]5000 (M_w=5000)showed slightly inhibitory effects on the proliferation and differentiation. In the case of the poly (L-lactic acid-co- epsilon -caprolactone)_(25) 10000[P(LA-CL)_(25) 10000] (M_w= 10000), the differentiation was inhibited at 7.5 mu g/ml to the levels of about 50 percent of the controls. But, P(LA-CA)_(50) 18000 (M_w=18000) showed weakly inhibitory action on the differentiation of midbrain cells in comparison with P(LA-CA)_(25) 10000. The inhibitory activity of these polymers on neuronal cell differentiation were in the following order: P(LA-CA)_(25) 10000 > PGA3000> P(LA-CA)_(50) 18000= PLLA5000. The catalyst of SnCl_2 showed the strongest inhibitory action on the midbrain cell differentiation among test chemicals.
机译:测定代表性可生物降解聚合物,以澄清使用中脑细胞分化系统用作神经再生材料的性质。对于聚乙醇酸[PGA],通过PGA3000(M_W = 3000)抑制中脑细胞的增殖和分化均依赖性抑制。聚(L-乳酸)[PLLA] 5000(M_W = 5000)对增殖和分化略微抑制作用。在聚(L-乳酸 - ε-己内酯)_(25)10000 [P(La-Cl)_(25)10000](M_W = 10000)中,在7.5μg的情况下抑制分化/ ml到约50%的对照的水平。但是,p(la-ca)_(50)18000(m_w = 18000)与p(la-ca)_(25)10000相比,对中脑细胞的分化显示弱抑制作用。这些聚合物的抑制活性神经元细胞分化按以下顺序:P(La-CA)_(25)10000> PGA3000> P(La-CA)_(50)18000 = PLLA5000。 SnCl_2的催化剂显示出测试化学品中的中脑细胞分化的最强抑制作用。

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