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The Effects of Anodic Oxide Films Produced by β-Glycerophosphate and Calcium Acetate Anodizing on Attachment and Spreding of Osteobiaste-like Cell

机译:β-甘油磷酸盐和醋酸钙阳极氧化氧化钙氧化钙氧化钙氧化钙对骨质缺血剂的附着和渗透的影响

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The effect of anodic oxide films produced by β-glycerophosphate (β-GP) and calcium acetate (CA) anodizing on osteoblast-like cell attachment and spreading were evaluated in this study. Anodic oxide films were produced in different conditions: Group 1, 0.02 M β-GP and 0.2 M CA; Group 2, 0.03 M β-GP and 0.2 M CA; Group 3, 0.03 M β-GP and 0.2 M CA. Anodic oxide surface was significantly rougher in comparison to the control untreated titanium surfaces, and the surface roughness and composition of phosphate and oxide increased as the concentration of β-GP was increased. There was no significant difference in the cell viability when cells were cultured on the control or anodized surface using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Scanning electron micrographs revealed more spread cells on the anodized surface than on the smooth control surface. In conclusion, we suggested that the positive effects of anodized surfaces produced by β-GP and CA on spreading of osteoblast-like cells may be the result of the difference of surface roughness and amount of Ca and P in the oxide layer.
机译:通过β-甘油磷酸盐(β-GP)和醋酸钙氧化物(CA)阳极氧化在该研究中,评估了阳极氧化膜的疗效和氧化钙氧氧化氧氧化物。在不同的条件下生产阳极氧化膜:第1组,0.02Mβ-GP和0.2M Ca;第2组,0.03Mβ-GP和0.2米;第3组,0.03Mβ-GP和0.2米。与控制未处理的钛表面相比,阳极氧化物表面显着变得更加粗糙,并且随着β-GP浓度增加,磷酸盐和氧化物的表面粗糙度和组成增加。当使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴(MTT)测定,在对照或阳极氧化表面上培养细胞培养细胞时,细胞活力没有显着差异。扫描电子显微照片在阳极氧化表面上显示比光滑的控制表面更多的涂布电池。总之,我们建议β-GP和Ca产生的阳极氧化表面对成骨细胞样细胞扩散的正效应可以是氧化物层中的表面粗糙度和Ca和P的量差异的结果。

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