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首页> 外文会议>Congress of the International Pig Veterinary Society >USE OF PCR-RFLP AS AN ALTERNATIVE METHOD FOR HAEMOPHILUS PARASVIS GENOTYPING
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USE OF PCR-RFLP AS AN ALTERNATIVE METHOD FOR HAEMOPHILUS PARASVIS GENOTYPING

机译：使用PCR-RFLP作为血液血症促胰蛋毒基因分型的替代方法

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Control of Haemophilus parasuis infections depends on the accurate diagnosis of the agent, identification of the prevalent strains causing disease in the herd, management of the introduction of new strains into the herd and use of adequate vaccine programs. Diagnosis and identification of H. parasuis is usually based on the isolation of the organism from lesions and further characterization of isolates by serotyping and or genotyping. Isolation of H. parasuis from clinical samples has been reported as difficult and frequently unsuccessful. Unfortunately, all currently available typing methods for H. parasuis require the isolation and biochemical identification of the isolates. Recently a 16S rRNA gene-based PCR was developed to improve the diagnosisof H. parasuis infections. The PCR was able to detect the presence of H, parasuis in clinical samples even when isolation was not accomplished. Study of the restriction sites present within the PCR-amplified sequences from 12 H. parasuis reference strains demonstrated that these strains could be grouped into four PCR-RFLP types. In this study, we evaluated the use of PCR-RFLP as an alternative method for differentiation of H. parasuis isolates recovered from a field outbreak.
机译：寄生虫治疗寄生虫感染取决于药剂的准确诊断，鉴定群体中患有疾病的普遍菌株，管理新菌株进入畜群和使用充足的疫苗计划。普拉的诊断和鉴定通常基于生物体从病变的分离和通过血清型和或基因分型进一步表征分离物。从临床样品中分离p. parasuis的临床样本难以和经常不成功。不幸的是，所有目前可用的H.Parasuis的类型方法需要隔离和生化隔离鉴定。最近，开发了16S rRNA基因的PCR，以改善H.Parasuis感染的诊断。即使在未完成分离时，PCR能够检测临床样品中H的存在，临床样品中。从12小时的PCR扩增序列中存在的限制性位点的研究表明，这些菌株可以分为四种PCR-RFLP类型。在该研究中，我们评估了PCR-RFLP作为分化的替代方法，用于从田间爆发中恢复的普拉沙分离物。

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