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首页> 外文会议>International Wheat Genetics Symposium >Construction of contigs oi Aegihps tauschii genomic DNA fragments cloned in BAC and BiBAC vectors
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Construction of contigs oi Aegihps tauschii genomic DNA fragments cloned in BAC and BiBAC vectors

机译：Contigs oi aegihps tauschii基因组DNA片段在Bac和Bibac载体中克隆

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A high-throughput, fully automated, multi-color fluorescent fingerprinting technique for large-insert genomic DNA clones was developed and used to fingerprint 215,645 genomic fragments of an Aegihps tauschii line that is genetically very closely related to the D genome of Chinese Spring wheat (Triticum aestivum). The fragments were cloned in BAC and BiBAC vectors. The fingerprints were edited with a computer program (GenoPro filer) we developed and assembled into contigs with the FPC program. A totalof 10,035 contigs were obtained at a Sulston score of 1><10"30 and a tolerance of 0.4 bp, corresponding to ca. ;200 Mb. Some of the contigs have been anchored to wheat maps by hybridization of RFLP markers and mapped ESTs with BAC and BiBAC clones. A database including fingerprints, BAC and BiBAC clones, contigs, and integrated markers has been developed and is publicly accessible at http: //wheatdb. ucdavis.edu: 8080/wheatdb/.
机译：用于大嵌入基因组DNA克隆的高通量，全自动，多色荧光指纹识别技术，并用于AEGIPPS Tauschii系的指纹215,645基因组片段，其与中国春小麦的D基因组（ Triticum aestivum）。将片段克隆在Bac和Bibac载体中。使用我们开发并组装成FPC程序的计算机程序（Genopro Filer）编辑指纹。以1> <10“30的磺顿成绩获得10,035个葡萄片，对应于约30的耐受性0.4bp。; 200 MB。通过RFLP标记物杂交和映射EST的杂交已经将某些环节固定在小麦地图上使用BAC和BIBAC克隆。已经开发了一种包括指纹，BAC和BIBAC克隆，CONTIG和集成标记的数据库，并在HTTP：// WHEATDB上公开访问。UCDAVIS.edu：8080 / WHEATDB /。

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