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首页> 外文会议>International Wheat Genetics Symposium >Optimisation of factors for Agwbacterium-mediated genetic transformation of wheat (cv Florida).
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Optimisation of factors for Agwbacterium-mediated genetic transformation of wheat (cv Florida).

机译：优化抗杆菌介导的小麦遗传转化的因素（CV佛罗里达州）。

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Cereal species in general and wheat; in particular; have lagged behind dicots in their ability to be transformed by Agrobacterium and remain relatively genotype-dependent. We used the Agrobacterium strain AGLl harbouring the pGreen-based plasmid (pAL156) containing a T-DNA incorporating the bar gene and a modified uidA gene to investigate and optimise major variables thought to influence T-DNA delivery; plant regeneration and stable transformation in winter wheat Florida. Factors that produced significant differences in T-DNA delivery and regeneration included embryo size; duration of pre-culture; inoculation and co-cultivation; and the presence of acetosyringone and Silwet-L77 in the media. The protocol we developed allowed efficient T-DNA delivery and gave rise to morphologically normal; and fiilly fertile; stable transgenic plants. Transformation frequency ranged from 0.3-3.3%. Marker-gene expression and molecular analysis demonstrated that transgenes integrated into the wheat genome and were transmitted into progeny with Mendelian ratios.
机译：谷物种类一般和小麦;特别是;落后于分枝杆菌的能力，其能够被土壤杆菌转化并保持相对基因型依赖性。我们使用含有含有含有杆基因的T-DNA的Purreen基质粒（Pal156）的农杆菌菌株agll，并进行修饰的UIDA基因，以研究和优化思想影响T-DNA递送的主要变量;冬小麦佛罗里达植物再生和稳定的转化。产生T-DNA递送和再生的显着差异的因素包括胚胎尺寸;预文化的持续时间;接种和共同栽培;和培养基中的乙酰菌酮和Silwet-L77的存在。我们开发的方案允许有效的T-DNA递送并产生形态正常;和肥沃的;稳定的转基因植物。转化频率范围为0.3-3.3％。标记基因表达和分子分析表明，与孟德尔比率一起融入小麦基因组中的转基因。

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