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首页> 外文会议>International Wheat Genetics Symposium >Two classes of Mutator trans posable element-like sequences in the wild einkorn wheat.
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Two classes of Mutator trans posable element-like sequences in the wild einkorn wheat.

机译：野生Einkorn小麦中的两类突变脉状物反式可染色元素样序列。

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Mutator, a wide-spread DNA-type transposable element, was first identified in maize and has been extensively characterized. To study Mutator-like transposable elements (MULEs) in wheat, homologous clones were isolated from Triticum urartu using degenerate primers constmcted according to the conserved transposase sequences of maize MuDR and its rice orthologue OsMu. DNA fragments encoding a part of the transposase were obtained by PCR. Deduced amino acid sequences of all 29 amplified DNA fragments with the expected length showed significant homology with the corresponding maize and rice transposase sequences. Nucleotide sequences were divided into two clusters using the UPGMA method. Some members in both clusters possessed intact transposase sequences without interrupting stop codons. DNA gel blot analysis was conducted in the two wild einkom wheat and several wild and cultivated polyploid wheat using representative sequences of the two clusters as probes. Hybridization pattems showed that one cluster consisted of low-copy elements, while the other consisted of remarkably high-copy elements. The results show that A genome of the wild einkom wheat possesses at least two classes of MULEs that had greatly different transposition activities.
机译：突变剂，宽扩散的DNA型转换元件首先在玉米中鉴定，并已被广泛表征。为了研究小麦中的突变剂样转移元素（骡子），使用根据玉米Mudr的保守转座序列和水稻直蛋白OSMU的保守转座序列来从Triticum urartu中分离同源克隆。通过PCR获得编码转座酶一部分的DNA片段。推导出所有29个扩增的DNA片段的氨基酸序列，其预期长度与相应的玉米和水稻转子酶序列显示出显着的同源性。使用UPGMA方法将核苷酸序列分成两种簇。两个簇中的一些成员在不中断止挡密码子的情况下具有完整的转座酶序列。 DNA凝胶印迹分析在两种野生excinem小麦和几种野生和培养的多倍体小麦中进行，使用两种簇的代表序列作为探针。杂交模式表明，一个群集由低拷贝元素组成，而另一个群体由其他高拷贝元素组成。结果表明，野生Einkom小麦的基因组具有至少两类骡子，具有很大不同的转子活动。

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