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首页> 外文会议>Annual Meeting of the Japanese Association for Animal Cell Technology >EFFECT OF p-CASEIN ON CYTOKINE PRODUCTION OF MOUSE SPLEEN LYMPHOCYTES
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EFFECT OF p-CASEIN ON CYTOKINE PRODUCTION OF MOUSE SPLEEN LYMPHOCYTES

机译：p-catein对小鼠脾脏淋巴细胞细胞因子产生的影响

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We showed p-Casein derived from bovine milk dose-dependently enhanced interferon (IFN)-γ and tumor necrosis factor (TNF)-a productions but not interleukin (IL)-4 production by mouse spleen lymphocytes. Furthermore, β-Casein increased the percentageof CD4~+ T cell ratio but not CD8~+ T cell ratio. That is, β-Casein significantly increased CD4~+/CD8~+ ratio. It is supposed from these results that β-Casein activates CD4~+ T cells, especially Thl type cells. And, the effects of β-Casein on the mRNAexpressions of various cytokines in spleen lymphocytes were performed using RT-PCR method. RT-PCR analysis showed that spleen lymphocytes expressed the mRNA for IFN-γ, IL-2, IL-4, IL-6, IL-10 and IL-12p40 under Concanavalin A (ConA) stimulation. In addition, β-Casein stimulus increased the mRNA level of IFN-γ, IL-4 and IL-6 under ConA stimulation. And β-Casein stimulus increased the mRNA level of IL-12p40 under none stimulation. These results suggested that p-Casein activated not only IFN-γ production but also transcription activity of IFN-γ and spleen T cell response to the p-Casein is of the Thl type. Moreover, the binding of the β-Casein to spleen lymphocytes was assessed by flow cytometory. β-Casein was strongly bound to some spleen lymphocytes, which were expected to be P-Casein sensitive population.
机译：我们发现对 - 酪蛋白从牛奶剂量依赖性地增强干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α的制作，而是由小鼠脾脏淋巴细胞不白细胞介素（IL）-4生产的。此外，β酪蛋白增加了percentageof CD4 + T细胞比率而不是CD8 + T细胞的比率。也就是说，β酪蛋白显著增加CD4 + / CD8 +的比例。从这些结果假设β酪蛋白激活CD4 + T细胞，特别是的Th1型细胞。并且，使用RT-PCR法进行β酪蛋白对脾脏淋巴细胞的各种细胞因子的mRNAexpressions的影响。 RT-PCR分析表明，脾淋巴细胞表达下伴刀豆球蛋白A（刀豆）刺激mRNA表达的IFN-γ，IL-2，IL-4，IL-6，IL-10和IL-12p40。此外，β酪蛋白的刺激下的ConA刺激增加IFN-γ的mRNA水平，IL-4和IL-6。和β酪蛋白的刺激下无刺激增加IL-12p40的mRNA水平。这些结果表明，对 - 酪蛋白激活不仅IFN-γ的生产，而且还IFN-γ和脾T细胞应答于p酪蛋白是所述Th1型的转录活性。此外，β酪蛋白的结合到脾脏淋巴细胞通过流式细胞cytometory评估。 β酪蛋白被牢固地结合一些脾脏淋巴细胞，这预计是β-酪蛋白敏感的人群。

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