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首页> 外文会议>Annual Meeting of the National Mastitis Council >GENE EXPRESSION NETWORKS IN BOVINE MAMMARY TISSUE DURING A STREPTOCOCUS UBERIS INTRAMAMMARY INFECTION CHALLENGE
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GENE EXPRESSION NETWORKS IN BOVINE MAMMARY TISSUE DURING A STREPTOCOCUS UBERIS INTRAMAMMARY INFECTION CHALLENGE

机译：牛乳腺组织中的基因表达网络在链球菌患者内部感染攻击中的牛霉菌组织

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Mammary epithelial cells (MEC) have immunological functions that contribute to the initial response to an intramammary infection (IMI; 4). Studies have indicated that MEC secrete several cytokines (e.g., interleukin-8 [IL-8] and tumor necrosis factor-a [TNFA]), enzymes (e.g., lysozyme and inducible nitric oxide synthase), and antimicrobial proteins (e.g., lactoferrin) that contribute to the immune response during an IMI (4, 5). Determining the genomic-level responses of MEC during an IMI using microarray technology and quantitative real-time PCR (qPCR) could provide useful information on additional signals produced by the mammary gland. Data from our laboratory examined the gene expression profiles in liver tissue collected from cows during the transition period (3). Results showed positive correlations between circulating fatty acids and ketones and hepatic mRNA for acute phase proteins such as serum amyloid Al (SAA1). and genes associated with fatty acid oxidation such as peroxisome proliferators-activated receptor a (PPAR-a). These results provide evidence for a relationship between immune response of hepatocytes and energy metabolism. Therefore, the objectives of this study are to: determine gene network and pathway expression patterns in mammary tissue in response to IMI; and relate these with other physiological measurements associated with immune and/or metabolic responses to mastitis challenge.
机译：乳腺上皮细胞（MEC）具有免疫功能，其有助于初始反应嵌入型感染（IMI; 4）。研究表明，MEC分泌几种细胞因子（例如，白细胞介素-8 [IL-8]和肿瘤坏死因子-A [TNFA]），酶（例如溶菌酶和诱导型一氧化氮合酶）和抗微生物蛋白（例如，乳铁蛋白）这有助于IMI（4,5）期间的免疫应答。使用微阵列技术和定量实时PCR（QPCR）确定MEC在IMI期间的基因组级响应可以提供有关由乳腺产生的附加信号的有用信息。我们实验室的数据检测了在过渡期间（3）中从奶牛收集的肝脏组织中的基因表达谱。结果表明循环脂肪酸和酮酮和肝癌mRNA之间的正相关性，例如血清淀粉样蛋白（SAA1）。和与脂肪酸氧化相关的基因，例如过氧化物酶体增殖剂 - 活化受体A（PPAR-A）。这些结果为肝细胞和能量代谢的免疫应答之间的关系提供了证据。因此，本研究的目的是：确定乳腺组织中的基因网络和途径表达模式，响应于IMI;并将这些与其他与免疫和/或代谢反应相关的其他生理测量对乳腺炎挑战相关联。

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