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首页> 外文会议>Asian Society for Mitochondrial Research and Medicine >Effects of Glucose and -Tocopherol on Low-Density Lipoprotein Oxidation and Glycation
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Effects of Glucose and -Tocopherol on Low-Density Lipoprotein Oxidation and Glycation

机译：葡萄糖和糖酚对低密度脂蛋白氧化和糖化的影响

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Glycation of blood proteins is considered to be a major contributor to hyperglycemic complications in diabetes mellitus patients. In this study, we demonstrate the efficacy of -tocopherol in reducing low-density lipoprotein (LDL) oxidation and glycation in vitro. Native LDL isolated from healthy subjects was exposed to various concentrations of glucose and malondialdehyde (MDA) with or without -tocopherol enrichment for 7 days in sealed vacuum ampoules. The degree of glycation, copper-induced lag time, content of thiobarbituric acid-reactive substances (TBARS), and -tocopherol levels in LDL were then assessed. LDL lag time was significantly reduced with high levels of glucose and MDA. -Tocopherol enrichment dramatically inhibited the oxidation of LDL in the lag-time assay. However, the length of incubation time was inversely related to the LDL lag time. Longer incubation time resulted in shorter LDL lag time, with or without -tocopherol enrichment. The level of TBARS associated with LDL oxidation was highest in native, MDA-supplemented, and high-glucose samples. The -tocopherol levels were inversely related to glucose levels and incubation times. In conclusion, high-glucose concentrations heightened the oxidative susceptibility of LDL. -Tocopherol enrichment reduced this trend and prevented LDL from undergoing architectural modification.
机译：血液蛋白的糖化被认为是糖尿病患者糖尿病患者高血糖并发症的主要因素。在这项研究中，我们证明了-Tocophherol在减少低密度脂蛋白（LDL）氧化和体外糖糖的疗效。从健康受试者中分离的本机LDL暴露于各种浓度的葡萄糖和丙二醛（MDA），在密封真空安瓿中7天，富硒血液富集7天。然后评估甘露然化程度，铜诱导的滞后时间，硫酰脲酸反应性物质（TBAR）和-Tocopherol水平的含量。随着高水平的葡萄糖和MDA，LDL滞后时间显着降低。 -Tocopherol富集显着抑制LDL在滞后时间测定中的氧化。然而，孵育时间的长度与LDL滞后时间相反。较长的孵育时间导致较短的LDL滞后时间，有或没有-Tocophherol富集。与LDL氧化相关的TBAR水平在天然，MDA补充和高葡萄糖样品中最高。 -Tocophherol水平与葡萄糖水平和孵育时间呈偶联。总之，高葡萄糖浓度提高了LDL的氧化敏感性。 -Tocophherol富集降低了这种趋势并阻止了LDL正在进行建筑修改。

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