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首页> 外文会议>Asian Society for Mitochondrial Research and Medicine >Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Induces Apoptosis of Human Leukemic U937 Cells
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Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Induces Apoptosis of Human Leukemic U937 Cells

机译：果冻图（Ficus Avkeotsang Makino）瘦果酶抑制剂诱导人白血病U937细胞的凋亡

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The antitumor activity of pectinesterase inhibitor (PEI), a group of cationic polypeptides, from jelly fig (Ficus awkeotsang Makino) achene was first examined as a treatment for leukemia in this study. PEI displayed strong growth inhibition against human leukemic U937 cells via induction of apoptosis in a dose- and time-dependent manner. At a level of 50 μg/mL, PEI inhibited 90% of cell growth, and the concentration of PEI required to induce 50% of cell viability (LC50) was about 180 μg/mL. Meanwhile, cell cycle arrest at G2/M phase was observed when cells were incubated with 100 μg PEI/mL for 24 h. PEI displayed a dose-dependent influence on mitochondria transmembrane potential (MTP, ΔΨm) of cells when detected by a flow cytometry. MTP of more than 50% cells was reduced when cells were incubated with PEI at levels higher than 50 μg PEI/mL for 24 h. In addition, PEI upregulated caspase-3 activity. Taken together, PEI potently inhibited the proliferation of human leukemic U937 cells via cell cycle arrest and apoptosis in association with MTP reduction and caspase-3 activation, respectively, and showed therapy potential for U937 cells.
机译：第一次检查果冻无花果（Ficus Avceotsang Makino）瘦果蛋白，一组阳离子多肽的抗肿瘤活性，是本研究中白血病的治疗方法。 PEI通过以剂量和时间依赖性的方式诱导细胞凋亡，对人白血病U937细胞进行强烈的生长抑制。在50μg/ ml的水平下，PEI抑制了90％的细胞生长，诱导50％的细胞活力所需的PEI浓度为约180μg/ ml。同时，当将细胞与100μgPEI/ ml温育24小时时，观察到G2 / M相的细胞周期停滞。当通过流式细胞术检测到时，PEI对细胞的线粒体跨膜电位（MTP，Δψm）显示了剂量依赖性影响。当用高于50μgPE/ mL的水平孵育细胞时，将细胞与24小时水平温育细胞，减少了超过50％细胞的MTP。此外，PEI上调的Caspase-3活性。在一起，PEI分别通过细胞周期停滞和细胞凋亡，分别与MTP还原和Caspase-3激活相关联的细胞循环骤停和细胞凋亡，并表现出U937细胞的治疗潜力，Pei Potely抑制了人白血病U937细胞的增殖。

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