Antioxidant N-Acetylcysteine Blocks Nerve Growth Factor-Induced H_2O_2/ERK Signaling in PC12 Cells

机译：抗氧化剂N-乙酰琥珀嵌段阻断神经生长因子诱导的PC12细胞中的H_2O_2 / ERK信号传导

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We investigated whether H_2O_2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H_2O_2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H_2O_2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H_2O_2/ERK signaling in PC12 cells.

机译：我们研究了H_2O_2，超氧化物和ERK是否参与神经生长因子（NGF）引起的信号级联，以及抗氧化剂N-乙酰半胱氨酸（NAC）调节这些NGF诱导的反应。在含有NGF或载体的培养基中培养PC12细胞，或没有NAC预处理，通过流式细胞术和Western印迹评估细胞内H_2O_2和超氧化物水平和磷酸化ERK的量。我们发现NGF增加细胞内H_2O_2浓度和活化的ERK，但未影响细胞内超氧化物水平。此外，NAC抵消了这些NGF诱导的反应。这些发现表明NAC阻断了PC12细胞中的NGF诱导的H_2O_2 / ERK信号。

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《Asian Society for Mitochondrial Research and Medicine》|2005年||共13页
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LIANG-YO YANG; WUN-CHANG KO; CHUN-MAO LIN; JIA-WEI LIN; JEN-CHINE WU; CHIEN-JU LIN; HUEY-HWA CHENG; CHWEN-MING SHIH;
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中图分类 R361-532;
关键词
antioxidant; N-acetylcysteine; PC12 cells; ERK; NGF; hydrogen peroxide; reactive oxygen species;

机译：抗氧化剂;n-乙酰琥珀酸;PC12细胞;ERK;ngf;过氧化氢;活性氧;

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1. Antioxidant N-Acetylcysteine Blocks Nerve Growth Factor-Induced H_2O_2/ERK Signaling in PC12 Cells [J] . LIANG-YO YANG, WUN-CHANG KO, CHUN-MAO LIN, Annals of the New York Academy of Sciences . 2005,第May期

机译：抗氧化剂N-乙酰半胱氨酸阻滞PC12细胞中神经生长因子诱导的H_2O_2 / ERK信号传导。
2. Global Expression Analysis Identified a Preferentially Nerve Growth Factor-induced Transcriptional Program Regulated by Sustained Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase (ERK) and AP-1 Protein Activation during PC12 Cell Differentiation [J] . Steven Mullenbrock, Janki Shah, Geoffrey Cooper The Journal of biological chemistry . 2011,第52期

机译：全局表达分析鉴定了通过持续的丝裂原激活的蛋白激酶/细胞内信号调节激酶（ERK）和PC12细胞分化期间的持续丝裂原激活蛋白激酶/细胞外信号调节激酶（ERK）和AP-1蛋白激活调节的过神经生长因子诱导的转录程序
3. 15d-prostaglandin J2 enhancement of nerve growth factor-induced neurite outgrowth is blocked by the chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells (CRTH2) antagonist CAY10471 in PC12 cells [J] . HatanakaM., ShibataN., ShintaniN., Journal of pharmacological sciences. . 2010,第1期

机译：15d-前列腺素J2增强神经生长因子诱导的神经突增生被PC12细胞中T型辅助2型细胞（CRTH2）拮抗剂CAY10471表达的趋化性受体-同源分子所阻断
4. Antioxidant N-Acetylcysteine Blocks Nerve Growth Factor-Induced H_2O_2/ERK Signaling in PC12 Cells [C] . LIANG-YO YANG, WUN-CHANG KO, CHUN-MAO LIN, Asian Society for Mitochondrial Research and Medicine . 2005

机译：抗氧化剂N-乙酰琥珀嵌段阻断神经生长因子诱导的PC12细胞中的H_2O_2 / ERK信号传导
5. The regulation of annexin II gene expression and function during nerve growth factor-induced differentiation of PC-12 cells. [D] . Jacovina, Andrew Thomas. 2000

机译：神经生长因子诱导PC-12细胞分化过程中Annexin II基因表达和功能的调节。
6. Inhibition of Nerve Growth Factor-Induced Neurite Outgrowth from PC12 Cells by Dexamethasone: Signaling Pathways through the Glucocorticoid Receptor and Phosphorylated Akt and ERK1/2 [O] . Kazuki Terada, Yoshitsugu Kojima, Takayuki Watanabe, -1

机译：地塞米松抑制PC12细胞神经生长因子诱导的神经突增生：糖皮质激素受体和磷酸化Akt和ERK1 / 2的信号通路。
7. Inhibition of nerve growth factor-induced neurite outgrowth from PC12 cells by dexamethasone: signaling pathways through the glucocorticoid receptor and phosphorylated Akt and ERK1/2. [O] . Kazuki Terada, Yoshitsugu Kojima, Takayuki Watanabe, 2014

机译：地塞米松抑制神经生长因子诱导的pC12细胞神经突向外生长：通过糖皮质激素受体和磷酸化akt和ERK1 / 2的信号通路。

Antioxidant N-Acetylcysteine Blocks Nerve Growth Factor-Induced H_2O_2/ERK Signaling in PC12 Cells

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