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首页> 外文会议>European Workshop on Equine Nutrition >The use of frozen equine faeces to assess the nutritional quality of forages using an in vitro gas production technique
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The use of frozen equine faeces to assess the nutritional quality of forages using an in vitro gas production technique

机译：使用冷冻马粪便使用体外气体生产技术评估锻造的营养质量

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The in vitro gas production technique (I VGPT) is used to evaluate the nutritional quality for forages. The methodology for the I VGPT uses freshly collected microbial inoculum as an alternative to caecal fluid, but freshly voided faces cannot alwaysbe obtained. Using frozen faeces as inocula for the IVGPT would provide flexibility to this technique. However, information on using frozen inocula in gas production (GP) studies is limited. The aim of this study was to evaluate the use of frozen equinefaeces as inocula for an IVGPT to assess the nutritional quality of forages. Two in vitro experiments were conducted. In experiment 1, high-temperature dried alfalfa (HTA) and mature grass hay (GH) were incubated with a faecal inoculum prepared either fresh (Fr) or following storage at -20 °C for 7 days (F7). In experiment 2, HTA, GH, high-temperature dried grass (HTG), and unmolassed sugar beet pulp (SB) were incubated with an inoculum prepared either fresh (Fr) or following storage at -20 °C for 24hours (F24). In both experiments, GP values revealed an interaction (P<0.05) between inocula and substrate. GP from HTA was less affected by inocula source compared to all other substrates. In experiment 1, total GP was reduced by 33% for GH inoculated with F7, compared to 3% for HTA, whilst in experiment 2, total GP was reduced by 47, 37, 14 and 8% for GH, HTG, SB and HTA, respectively when inoculated with F24 compared to fresh faeces. The rate of GP was also affected by inocula source in both experiments. The use of frozen feaces as source of inocula affects the extent and rate of substrate fermentation in vitro, which appears to be dependent upon the nature of substrate assessed.
机译：体外气体生产技术（I VGPT）用于评估饲料的营养质量。 I VGPT的方法使用新鲜收集的微生物接种物作为粘型流体的替代方法，但不能总是获得新的空隙面。使用冷冻粪便作为IVGPT的ineCula将为这种技术提供灵活性。然而，有关在天然气生产（GP）研究中使用冷冻接种的信息有限。本研究的目的是评估使用冷冻的EquineFaeCes作为Inocula以评估饲料的营养质量。进行了两种体外实验。在实验1中，将高温干燥的苜蓿（HTA）和成熟的草草（GH）与在-20℃下在-20℃下储存7天（F7）中制备的粪便接种物或在-20℃下储存。在实验2中，将HTA，GH，高温干草（HTG）和未溶解的甜菜纸浆（SB）与在-20℃下在-20℃下储存的占状内或在-20℃下储存24hours（F24）。在两个实验中，GP值显示接种物和底物之间的相互作用（P <0.05）。与所有其他基材相比，来自HTA的GP对接种来源的影响较小。在实验1中，通过F7接种的GH减少了33％的33％，而HTA的3％相比，在实验2中，GH，HTG，SB和HTA的总GP减少了47,37,14和8％分别与F24接种与新鲜粪便接种时。在两个实验中，GP的速率也受到因子源的影响。使用冷冻的血液作为接种源的来源会影响体外底物发酵的程度和速率，这似乎取决于评估的基材的性质。

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