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首页> 外文会议>International Congress of Andrology >Evidence for Membrane Raft formation during low temperature incubation of Macaque (Macaca mulatta) Spermatozoa
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Evidence for Membrane Raft formation during low temperature incubation of Macaque (Macaca mulatta) Spermatozoa

机译：猕猴（Macaca Mulatta）精子低温孵育期间膜筏形成的证据

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Lipid rafts play important roles in signal transduction and other cellular processes. It has been described that sperm subjected to cryopreservation techniques undergo some physiological modifications that result in similar, but not identical changes in the sperm membrane during capacitation in vitro. This phenomenon is currently known as "cryocapacitation". We examined the formation of raft aggregates in the plasma membrane of macaque spermatozoa incubated in basic media at 4°C for 1 hour and compare these samples with the appropriate controls. In cell membranes, individual rafts are as small as 70-90 nm but the aggregates are much larger domains and can be seen with light microscopy with the aid of DiI-C18 stain that preferentially partitions into ordered lipid domains. Raft aggregates were clearly observed in samples subjected to low temperatures (4°C) similar to those observed in the in vitro capacitated samples. The lipid composition of these raft domains showed a characteristic increase in the content of sphingomyelin and cholesterol compared to the whole plasma membrane analyzed by high-performance thin-layer chromatography. These data suggest that raft aggregation is a dynamic physiological event found in macaque sperm plasma membranes triggered by thermal stress conditions and capacitation.
机译：脂质筏在信号转导和其他细胞过程中起重要作用。已经描述了对冷冻保存技术进行的精子经历了一些生理修饰，其导致在体外电容过程中的精子膜中的相似性但不相同的变化。这种现象当前称为“冷冻胶合”。我们检查了在4℃下在碱性介质中孵育的猕猴的质膜中的筏聚集体的形成1小时，并将这些样品与适当的对照进行比较。在细胞膜中，单个筏小至70-90nm，但聚集体是更大的结构域，并且可以通过光学显微镜观察借助于DII-C18污渍，优先分配到有序的脂质结构域中。在与在体外电容样品中观察到的低温（4℃）的样品中清楚地观察到筏骨料。与通过高性能薄层色谱分析的全部血浆膜相比，这些筏区域的脂质组合物显示出鞘磷脂和胆固醇的含量增加。这些数据表明，RAFT聚集是在由热应力条件和电容引发的猕猴精子膜中发现的动态生理事件。

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