The mechanical folding and unfolding behaviors of single graphene flakes on flat substrates were investigated by using atomic force microscopy nanomanipulation techniques. The nanomechanical measurements show that individual few-layer graphene sheets can be mechanically folded in both fold-up and bucking delamination modes, the latter of which leads to accordion-shape self-folded graphene. The results reveal that the bending stiffness of two to six layers graphene follows a square-power relationship with its thickness.
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