首页> 外文会议>International Congress of Photosynthesis >FRAGMENTATION OF THE LARGE SUBUNIT OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE BY REACTIVE OXYGEN SPECIES OCCURS IN VIVO
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FRAGMENTATION OF THE LARGE SUBUNIT OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE BY REACTIVE OXYGEN SPECIES OCCURS IN VIVO

机译:通过反应性氧物种的核糖糖-1,5-双磷酸羧酶/氧酶的大亚基的破碎发生在体内

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Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC4.1.1.39) is a bifunctional enzyme that catalyzes photosynthetic CO_2 assimilation and photoiespiratory carbon oxidation in the stroma of chloroplasts. The amounts of the enzyme can be a limiting factor for the light-saturated photosynthesis in present-day air and accounts for 12-35% of total leaf protein in C_3 plants. During senescence or under environmental stress conditions, Rubisco is gradually or rapidly degraded, and its nitrogen is remobilized and translocated into growing organs. Therefore, Rubisco degradation is associated with both photosynthetic capacities and nitrogen economy in leaves. However, the degradation process of Rubisco in leaves is yet not known. During leaf senescence and under oxidative-stress conditions, the production of reactive oxygen species (ROS) in chloroplasts is stimulated. It is suggested that ROS act as the trigger of a protein degradation. Actually, the direct fragmentation of the large subunit (LSU) of Rubisco by ROS has previously been shown in vitro (Ishida et al 1997, 1998, 1999, Luo et al 2002). However, it is not known whether ROS could cause the fragmentation of Rubsco-LSU in vivo. In the present study, we examined whether the direct fragmentation of the Rubisco-LSU by ROS occurs in cucumber leaf discs under light-chilling stress.
机译:核糖糖-1,5-双磷酸羧酶/氧气(Rubisco; EC4.1.1.39)是一种双官能酶,其催化光合作用CO_2同化和光学血管碳氧化在叶绿体的基质中。酶的量可以是当前空气中光饱和光合作用的限制因素,并且占C_3植物中总叶片蛋白的12-35%。在衰老期间或在环境压力条件下,Rubisco逐渐或迅速降解,其氮气被重新化并易于生长的器官。因此,Rubisco降解与叶片的光合能力和氮经济性有关。然而,Rubisco在叶片中的降解过程尚未知道。在叶片衰老和在氧化胁迫条件下,刺激叶绿体中反应性氧物质(ROS)的产生。建议ROS充当蛋白质降解的触发。实际上,ROS的大亚基(LSU)的直接碎片以前已经在体外显示(Ishida等1997,1998,1999,Luo等人2002)。但是,尚不清楚ROS是否可能导致体内rubsco-lsu的碎片。在本研究中,我们检查了在轻微的胁迫下,在黄瓜叶片椎间盘中发生ruOSco-lsu的直接碎片。

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