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A Living Cell Array (LCA) for Multiparameter Cell Metabolism Studies

机译:用于多分钟细胞代谢研究的活细胞阵列(LCA)

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A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.
机译:介绍了用于分析生物电池阵列的平台概念架构。进行的初始实验显示了实时用于单细胞多级计测量的概念设计。作为第一个参数,广泛的开发和实验已经在传感器上进行,该传感器将测量单细胞氧气消耗。已经实现了芯片制造和传感器沉积的晶片级处理,使得使用一次性芯片的自动化系统的未来目标可能成为可能。已经测试了氧传感器,用于长期粘附到水性环境中的基材,与细胞类型的生物相容性,以及对局部氧浓度的小变化的敏感性。制成测试装置以验证金箔屏障作为氧封印的使用,并显示出密封区域的良好分离。

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