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Interactions between Agrobacterium radiobacter and dicot and monocot plants

机译:农杆菌辐射杆菌与单焦炭和单子叶植物之间的相互作用

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Bacteria of the genus Agrobacterium are known to infect di- and monocotyledonous plants and to inducetumors on these plants. Agrobacterium tumefaciens transfers a part of its Ti plasmid DNA (T-DNA) intoplant cells, thus initiating proliferation of host cells. Before the T-DNA is transferred, bacteria mustattach to the plant cell surface. All known genes participating in the attachment process (eel, att, chvA,chvB, exoC) are localized on the chromosome (1). A. tumefaciens mutants, defective in attachment, areavirulent, suggesting that agrobacterial attachment to the plant cell surface plays an important role in theplant-microbe interaction. It is known that A. radiobacter is a natural antagonist of A. tumefaciens. A.radiobacter strain 5D-1 was isolated from wheat roots after surface sterilization in Russia (Saratovregion) in 1986 (2) and re-isolated from wheat roots in 1994 as A. radiobacter 5D-2 (3). Both strainsshowed similar culture characteristics and patterns of EcoRI, HindIII-DNA restriction fragments andgave a positive reaction with 5D-1 strain antibodies. Thus A. radiobacter possibly belongs torhizosphere-associative bacteria.
机译:已知农杆菌属的细菌感染这些植物上的二胞膜和单子叶植物和紫外线。农杆杆菌突发胶剂转移其Ti质粒DNA(T-DNA)间隔细胞的一部分,从而引发宿主细胞的增殖。在将T-DNA转移之前,细菌Mustattach对植物细胞表面。所有已知的参与附着过程(鳗鱼,ATT,CHVA,CHVB,EXOC)的基因均在染色体上定位(1)。 A. Tumefaciens突变体,附着的缺陷,面部吞咽,表明对植物细胞表面的农杆菌附着在植物 - 微生物相互作用中起重要作用。众所周知,A.Axibobacter是A.Amefaciens的天然拮抗剂。 1986(2)在俄罗斯(Saratovregaregion)的表面灭菌后,从小麦根中分离出A.radiobacter菌株5D-1,并在1994年的小麦根作为A.辐射杆菌5D-2(3)。菌株的相似培养特征和eCORI的图案,HindIII-DNA限制性片段,凝结与5d-1应变抗体的正反应。因此,A.辐射杆菌可能属于托丘圈缔合的细菌。

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