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首页> 外文会议>International Colloquium on Paratuberculosis >Use of Mycobacterium avium ssp. paratuberculosis 70 kD heat shock protein as a subunit vaccine against bovine paratuberculosis
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Use of Mycobacterium avium ssp. paratuberculosis 70 kD heat shock protein as a subunit vaccine against bovine paratuberculosis

机译：使用分枝杆菌SSP。 Paratuberculosis 70 kd热休克蛋白作为亚基疫苗靶向牛副植物

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Introduction: Paratuberculosis. caused by Mycobacterium avium ssp. paratuberculosis (MAP), constitutes a global threat to ruminant health. Eradication of paratuberculosis could be aided with a vaccine. The use of current whole bacterin vaccines is limited due to lack of efficacy and interference with tuberculosis eradication strategies. In the current study we evaluated recombinant MAP heat shock protein 70kD (Hsp70) as a subunit vaccine candidate. Materials & Methods: In total 40 female calves were randomly assigned to one of 4 experimental groups (n=10 each) which were orally infected (groups G3 and G4), or left uninfected (groups G1 and G2). Calves of G2 and G4 were vaccinated with 100 ug of Hsp70 in DDA adjuvant at day 0 and 365 of the experiment. Blood samples were taken every 2 weeks, and used for isolation of lymphocytes, and serological analysis. Antigen specific activation of lymphocytes was evaluated in flowcytometry and IFN-gamma Elispot. Fecal samples were taken for culture of MAP 7 times during the first 2 years of the experiment, in which the calves were raised and fed conventionally.
机译：介绍：paratuberculosis。由分枝杆菌SSP引起的。对反刍动物健康构成全球威胁的paratuberculosis（地图）。可以援助疫苗的消除疫苗。由于缺乏疗效和干扰结核病根除策略，使用当前整个细菌疫苗的使用受到限制。在本研究中，我们评估重组地图热休克蛋白70KD（HSP70）作为亚基疫苗候选者。材料和方法：总共40个雌性小牛被随机分配给4个实验组（n = 10各自）中的一种，它们是口服感染（组G3和G4），或者离开未感染的（组G1和G2）。在第0天和第365天，在DDA佐剂中用100μgHsp70接种G2和G4的犊牛。每2周服用血样，并用于分离淋巴细胞，以及血清学分析。在流式细胞术和IFN-Gamma ELISPOT中评价抗原特异性淋巴细胞的活化。在实验的前2年期间，将粪便样品用于MAP 7次，其中牛犊饲养并常规喂养。

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