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首页> 外文会议>International Colloquium on Paratuberculosis >Ag85 homologues from culture filtrate of Mycobacterium avium subsp. paratuberculosis are immunodominant Th1-type antigens in experimentally infected cattle
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Ag85 homologues from culture filtrate of Mycobacterium avium subsp. paratuberculosis are immunodominant Th1-type antigens in experimentally infected cattle

机译：AG85来自分枝杆菌患者的培养滤液的同源物。对副伞菌在实验感染牛中的免疫肿瘤Th1型抗原

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The characterization of protective antigens is essential for the development of an effective, sub-unit based vaccine against paratuberculosis. Surface-exposed and secreted antigens, present abundantly in mycobacterial culture filtrate (CF), are among the well-known protective antigens for M. tuberculosis and M. bovis. In order to study the vaccine potential of CF in Johne's disease, M. avium subsp. paratuberculosis (MAP) ATCC 19698 was first adapted to grow as a surface pellicle on synthetic, protein-free Sauton medium and CF was prepared from 4 week old cultures. Strong proliferative and IFN-gamma responses against MAP CF could be detected as early as 4 weeks post infection (p.i.) in PBMC cultures from a bull infected by the intravenous route with 10~8 CFU of MAP ATCC 19698. Analysis of the immune response against MAP CF separated by electro-elution demonstrated that these early bovine T cell responses were mostly directed against fractions in the 30 kDa region, containing homologues of the Ag85 complex of BCG with well-documented vaccine potential. We conclude that homologues of the antigen 85 family are immunodominant antigens of MAP in experimentally and naturally infected cattle, inducing strong proliferative and IFN-gamma T cell responses. Their potential as possible vaccine candidates remains to be determined.
机译：保护性抗原的表征对于对抗伞菌的有效亚单位的疫苗发育至关重要。表面暴露和分泌的抗原在分枝杆菌培养物滤液（CF）中呈现大规模的，是拟已知的患有结核病和棉絮的保护抗原。为了研究Johne疾病中CF的疫苗潜力，M. Avium Subsp。 Paratuburisculosis（MAP）ATCC 19698首先适用于在合成的表面薄膜上生长，可使蛋白质培养基培养基和CF从4周龄培养物中制备。可以早于静脉内途径的静脉内途径（PI）在PBMC培养物中的4周龄（PI）在静脉内途径19698的静脉内途径中的培养物（PI）中的4周内检测到对映射CF的强烈增殖和IFN-gamma反应。分析免疫应答通过电洗采分离的地图CF证明了这些早期的牛T细胞应答大多是针对30kDa区域的级分，含有BCG的Ag85络合物的同源物，具有良好地记录的疫苗潜力。我们得出结论，抗原85家族的同源物是在实验和天然感染的牛中的地图的免疫模数抗原，诱导强增殖性和IFN-Gamma T细胞反应。他们作为可能的疫苗候选人的潜力仍有待确定。

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