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Screening of anti-peptide antibodies in vitro to identify potential cattle tick vaccine antigens

机译：体外筛选抗肽抗体以鉴定潜在的牛蜱疫苗抗原

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A reverse vaccinology or genome-based approach to develop an anti-cattle tick (Rhipicephalus (Boophilus) microplus) vaccine resulted in the identification of B cell binding epitopes from the predicted protein sequences from an EST library (13,643 ESTs), subtraction library clones (feeding and host sensing stages) and microarrays experiments conducted using ticks collected from susceptible and resistant cattle. Seven hundred and forty eight synthetic peptides were designed to target predicted B cell epitopes (BepiPred) conserved for 48 protein families and 99 hypothetical single predicted proteins (representing 248 ESTs) and 78 were selected for antibody generation in sheep. Antibodies were fed to semi-engorged female ticks in vitro to determine potential effects on fecundity to identify suitable antigens for cattle trials.
机译：逆向疫苗学或基于基于基于基于基于基于基于基于抗牛蜱的方法（Rapicephalus（Boophilus）疫苗疫苗导致来自EST文库（13,643 EST）的预测蛋白序列的B细胞结合表位，减法库克隆（ 喂养和宿主感测阶段）和微阵列使用从易受影响和抗性牛收集的蜱进行的微阵列实验。 设计为靶向预测的B细胞表位（Bepipred）保守48个蛋白质家族的靶向B细胞表位（Bepipred），并选择99个假想的单个预测蛋白（代表248 EST）和78用于绵羊的抗体产生。 将抗体进料至体外半孔的雌性蜱，以确定对繁殖力的潜在影响，以确定牛试验的合适抗原。

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