• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Lancefield International Symposium on Streptococci and Streptococcal Diseases >The contribution of serum opacity factor to group A streptococcal epithelial cell invasion
【24h】

The contribution of serum opacity factor to group A streptococcal epithelial cell invasion

机译：血清不透明因子对组链球菌上皮细胞侵袭的贡献

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Serum opacity factor (SOF) is a bifunctional cell surface protein expressed by 40-50% of group A streptococcal (GAS) strains comprised of a C-terminal domain that binds fibronectin and an N-terminal domain that mediates opaciiication of mammalian sera. SOF is co-transcribed in a two-gene operon with another fibronectin-binding protein, SfbX. We compared the ability of an SOF(+) wild-type (WT) serotype M49 GAS strain and isogenic mutants lacking SOF or SfbX to invade cultured Hep-2 human pharyngeal epithelial cells. Elimination of SOF led to a significant decrease in Hep-2 intracellular invasion while loss of SfbX had minimal effect. The hypoinvasive phenotype of the SOF(-) mutant could be restored upon complementation with the sof gene on a plasmid vector, and heterologous expression of so/49 in Ml GAS or Lactococcus lactis conferred marked increases in Hep-2 cell invasion. Studies using a mutant so/49 in which the fibronectin-binding domain had been deleted in-frame confirmed that the C-terminal domain of SOF contributes to cellular invasion independent of the fibronectin-binding domain.
机译：血清不透明度因子（SOF）是由40-50％的基团的双官能细胞表面蛋白组成，所述链球菌（气体）菌株组成，所述链球菌（气体）菌株包含结合纤连蛋白的C-末端结构域和介导哺乳动物血清的不透明度的N-末端结构域。 SOF是在两基因操纵子与另一个纤连蛋白结合蛋白，SFBX共转录。我们比较了SOF（+）野生型（WT）血清型M49气体菌株和缺乏SOF或SFBX的缺乏培养的HEP-2人咽部上皮细胞的能力。消除SOF导致HEP-2细胞内侵袭的显着降低，同时SFBX的损失效果最小。可以在与质粒载体上的SOF基因互补后恢复SOF（ - ）突变体的催眠表型，并且SO / 49在ML气体或乳乳球菌乳酸中的异源表达赋予HEP-2细胞侵袭的标记增加。使用突变体SO / 49的研究，其中已缺少纤维素结合结构域的框架证实，SOF的C末端域有助于与纤连蛋白结合结构域无关的细胞侵袭。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号